WB
Western blot analysis using PSAP antibody against Jurkat(1),C2C12(2),P19(3),Huvec(4),Mcf-7(5),A431(6),Pc-12(7) cell,Rat heart tissue(8) .IHC
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissues using PSAP antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human colon cancer tissues using PSAP antibody with DAB staining.| Product Name | Anti-PSAP antibody-poly-HRP |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Conjugation | poly-HRP |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:200-1:2000 IHC:1:100-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Antigen peptide affinity chromatography |
| Gene Symbol | PSAP |
|---|---|
| Gene Synonyms | GLBA PARK24 PSAPD SAP1 SAP2 SGP-1 SGP1A |
| Gene Full Name | prosaposin |
| Gene Summary | This gene encodes a highly conserved preproprotein that is proteolytically processed to generate four main cleavage products including saposins A, B, C, and D. Each domain of the precursor protein is approximately 80 amino acid residues long with nearly identical placement of cysteine residues and glycosylation sites. Saposins A-D localize primarily to the lysosomal compartment where they facilitate the catabolism of glycosphingolipids with short oligosaccharide groups. The precursor protein exists both as a secretory protein and as an integral membrane protein and has neurotrophic activities. Mutations in this gene have been associated with Gaucher disease and metachromatic leukodystrophy. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Feb 2016] |
| Alternative Names | co-beta glucosidase precursor precursor of saposins proactivator polypeptide prosaposin prosaposin (sulfated glycoprotein sphingolipid hydrolase activator) PSAP saposin-A saposin-B saposin-C saposin-D snoRNA MBII-198 sphingolipid activator protein sphingolipid activator protein-1 sphingolipid activator protein-2 sulfated glycoprotein 1 sulfated glycoprotein precursor |
| Molecular Weight(MW) | 58kDa(observed：70kDa) |
| Cellular Localization | Lysosome,Secreted |
| SwissProt ID | P07602 |
|---|
WB
Western blot analysis using PSAP antibody against Jurkat(1),C2C12(2),P19(3),Huvec(4),Mcf-7(5),A431(6),Pc-12(7) cell,Rat heart tissue(8) .
IHC
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissues using PSAP antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human colon cancer tissues using PSAP antibody with DAB staining.| Application Notes | WB:1:200-1:2000 IHC:1:100-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% PC300,40% Glycerol. |
