ICC/IF
Immunofluorescence analysis of NIH3T3 cells using C4 antibody (green). Blue: DAPI fluorescent DNA dye.Cells are fixed in 4% paraformaldehyde at room temperature for 15 minutes.| Product Name | Anti-C4 antibody-AF488 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Conjugation | AF488 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | C4A |
|---|---|
| Gene Synonyms | C4 C4-1 C4A2 C4A3 C4A4 C4A6 C4AD C4b C4S CO4 CPAMD2 RG |
| Gene Full Name | complement C4A (Chido/Rodgers blood group) |
| Gene Summary | This gene encodes the acidic form of complement factor 4, part of the classical activation pathway. The protein is expressed as a single chain precursor which is proteolytically cleaved into a trimer of alpha, beta, and gamma chains prior to secretion. The trimer provides a surface for interaction between the antigen-antibody complex and other complement components. The alpha chain is cleaved to release C4 anaphylatoxin, an antimicrobial peptide and a mediator of local inflammation. Deficiency of this protein is associated with systemic lupus erythematosus and type I diabetes mellitus. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. Varying haplotypes of this gene cluster exist, such that individuals may have 1, 2, or 3 copies of this gene. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2014] |
| Alternative Names | acidic C4 acidic complement C4 C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2 c4 propeptide C4A C4A anaphylatoxin C4AD CO4A complement C4-like complement C4A (Rodgers blood group) complement component 4 (within H-2S) complement component 4 gene 1 complement component 4A (Rodgers blood group) complement component 4B (Chido blood group) complement component 4B (Childo blood group) complement protein MHC class III region complement Rodgers form of C4 ZA |
| Molecular Weight(MW) | 192kDa |
| Cellular Localization | Secreted;Synapse;Cell projection, axon;Cell projection, dendrite |
| SwissProt ID | P0C0L4 |
|---|
ICC/IF
Immunofluorescence analysis of NIH3T3 cells using C4 antibody (green). Blue: DAPI fluorescent DNA dye.Cells are fixed in 4% paraformaldehyde at room temperature for 15 minutes.| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% PC300,40% Glycerol. |
