ICC/IF
Immunofluorescence analysis of NIH3T3 cells using AGRN antibody (green). Blue: DAPI fluorescent DNA dye.Cells are fixed in 4% paraformaldehyde at room temperature for 15 minutes.| Product Name | Anti-AGRN antibody-AF488 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Conjugation | AF488 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | AGRN |
|---|---|
| Gene Synonyms | AGR AGRIN CMS8 CMSPPD nmf380 |
| Gene Full Name | agrin |
| Gene Summary | This gene encodes one of several proteins that are critical in the development of the neuromuscular junction (NMJ), as identified in mouse knock-out studies. The encoded protein contains several laminin G, Kazal type serine protease inhibitor, and epidermal growth factor domains. Additional post-translational modifications occur to add glycosaminoglycans and disulfide bonds. In one family with congenital myasthenic syndrome affecting limb-girdle muscles, a mutation in this gene was found. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2015] |
| Alternative Names | agrin proteoglycan extracellular matrix proteoglycan laminin-binding domain RP23-139J18.2 synaptic basal lamina component |
| Molecular Weight(MW) | 217kDa |
| Cellular Localization | Secreted, extracellular space, extracellular matrix;Cell membrane |
| SwissProt ID | O00468 |
|---|
ICC/IF
Immunofluorescence analysis of NIH3T3 cells using AGRN antibody (green). Blue: DAPI fluorescent DNA dye.Cells are fixed in 4% paraformaldehyde at room temperature for 15 minutes.| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% PC300,40% Glycerol. |
