ICC/IF
Immunofluorescence analysis of U251 cells using MCM4 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Omnimabs® 594-Phalloidin.| Product Name | Anti-MCM4 antibody-AF488 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Conjugation | AF488 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Antigen peptide affinity chromatography |
| Gene Symbol | MCM4 |
|---|---|
| Gene Synonyms | 19G CDC21 CDC54 hCdc21 IMD54 mcdc21 Mcmd4 mKIAA4003 NKCD NKGCD P1-CDC21 |
| Gene Full Name | minichromosome maintenance complex component 4 |
| Gene Summary | The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. This gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks. Alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq, Jul 2008] |
| Alternative Names | CDC21 homolog homolog of S. pombe cell devision cycle 21 mini chromosome maintenance deficient 4 homolog minichromosome maintenance deficient 4 minichromosome maintenance deficient 4 homolog P1-CDC21 |
| Molecular Weight(MW) | 96kDa |
| Source | Rabbit |
| Cellular Localization | Nucleus;Chromosome;Cell membrane;Cytoplasm, cytosol |
| SwissProt ID | P33991 |
|---|
ICC/IF
Immunofluorescence analysis of U251 cells using MCM4 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Omnimabs® 594-Phalloidin.| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% PC300,40% Glycerol. |
