IHC
Confocal immunofluorescent analysis of COS cells expressing a Myc-tagged protein (left) and untreated (right), using Myc-Tag Mouse mAb (Alexa Fluor 647 Conjugate) (blue pseudocolor). Actin filaments have been labeled with DY-554 phalloidin (red)FC
Flow cytometric analysis of COS cells, untransfected (blue) or transfected with Myc-tagged Akt (green), using Myc-Tag Mouse mAb (Alexa Fluor 647 Conjugate).| Product Name | Anti-Myc-tag Antibody-AcalephFluor647 labled |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence of Myc-tag. |
| Conjugation | Alexa Fluor® 647 |
| Clonality | Monoclonal |
|---|---|
| Isotype | Ig2a,k |
| Host Species | Mouse |
| Tested Applications | FCIHC |
| IHC:1:100-400 FC:1:50 |
|
| Species Reactivity | All |
| Concentration | 1μg/μl |
| Purification | Affinity purified |
| Gene Symbol | MYC |
|---|---|
| Gene Summary | Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties. The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian cell systems |
IHC
Confocal immunofluorescent analysis of COS cells expressing a Myc-tagged protein (left) and untreated (right), using Myc-Tag Mouse mAb (Alexa Fluor 647 Conjugate) (blue pseudocolor). Actin filaments have been labeled with DY-554 phalloidin (red)
FC
Flow cytometric analysis of COS cells, untransfected (blue) or transfected with Myc-tagged Akt (green), using Myc-Tag Mouse mAb (Alexa Fluor 647 Conjugate).| Application Notes | IHC:1:100-400 FC:1:50 |
|---|
| Storage Instructions | 2-8℃ |
|---|---|
| Storage Buffer | Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA,40% Glycerol. |
