IP
For purification of N-terminal FLAG fusion proteins. Since binding is Ca2+ dependent, proteins can be eluted with a buffer containing EDTA, as well as by the standard methods using either FLAG peptide or glycine-HCl buffer, pH 3. ANTI-FLAG M1 does not bind to Met-FLAG fusion proteins, so this resin is not appropriate for purifying unprocessed, cytoplasmically expressed fusion proteins. Affinity gel is for calcium mediated purification of N-terminal FLAG fusion proteins. Elution - FLAG peptide, Glycine, pH 3.5 EDTA| Product Name | Anti-FLAG tag Antibody Conjugated Agarose Affinity Gel |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence of FLAG-tag |
| Conjugation | Agarose |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Mouse |
| Tested Applications | IP |
| capacity:≥0.6 mg/mL, gel binding capacity |
|
| Species Reactivity | Tag |
| Purification | Affinity purified |
| Gene Symbol | FLAG |
|---|---|
| Sequence | DYKDDDDK |
IP
For purification of N-terminal FLAG fusion proteins. Since binding is Ca2+ dependent, proteins can be eluted with a buffer containing EDTA, as well as by the standard methods using either FLAG peptide or glycine-HCl buffer, pH 3. ANTI-FLAG M1 does not bind to Met-FLAG fusion proteins, so this resin is not appropriate for purifying unprocessed, cytoplasmically expressed fusion proteins. Affinity gel is for calcium mediated purification of N-terminal FLAG fusion proteins. Elution - FLAG peptide, Glycine, pH 3.5 EDTA| Application Notes | capacity:≥0.6 mg/mL, gel binding capacity |
|---|
| Form | suspension |
|---|---|
| Storage Instructions | −20°C |
| Storage Buffer | Suspension of beaded agarose in 50% glycerol containing 10 mM sodium phosphate, 150 mM NaCl, pH 7.4, 0.02% (w/v) sodium azide |
