WB
Western blot analysis of various lysates using GNAI2 Rabbit mAb at 1:1000 dilution incubated atroom temperature for 1.5 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.IHC
Immunohistochemistry analysis of paraffinembedded Human liver using GNAI2 RabbitmAb at dilution of 1:20 (40x lens).High pressure antigen retrieval performedwith 0.01M Citrate buffer (pH 6.0) prior toIHC staining.| Product Name | GNAI2 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide. This information is considered to be commercially sensitive. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:1000-1:4000 IHC:1:20-1:200 |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GNAI2 |
|---|---|
| Gene Synonyms | GIP HG1C GNAI2B H_LUCA15.1 H_LUCA16.1 |
| Gene Full Name | G protein subunit alpha i2 |
| Gene Summary | The protein encoded by this gene is an alpha subunit of guanine nucleotide binding proteins (G proteins). The encoded protein contains the guanine nucleotide binding site and is involved in the hormonal regulation of adenylate cyclase. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2013] |
| Alternative Names | GIP HG1C GNAI2B H_LUCA15.1 H_LUCA16.1 GNAI2 |
| Molecular Weight(MW) | 40kDa(Observed band size:40kDa) |
| Cellular Localization | Cell membrane, Cytoplasm, Lipid-anchor, Membrane, centrosome, cytoskeleton, microtubule organizing center. |
WB
Western blot analysis of various lysates using GNAI2 Rabbit mAb at 1:1000 dilution incubated atroom temperature for 1.5 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.
IHC
Immunohistochemistry analysis of paraffinembedded Human liver using GNAI2 RabbitmAb at dilution of 1:20 (40x lens).High pressure antigen retrieval performedwith 0.01M Citrate buffer (pH 6.0) prior toIHC staining.| Application Notes | WB:1:1000-1:4000 IHC:1:20-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
