WB
Western blot analysis of c-Jun/JUN using anti-c-Jun/JUN antibody . The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: zebrafish head tissue lysates,Lane 2: whole female zebrafish tissue lysates,Lane 3: whole male zebrafish tissue lysates,Lane 4: zebrafish embryo tissue lysates.After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-c-Jun/JUN antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using ECL Plus Western Blotting Substrate . A specific band was detected for c-Jun/JUN at approximately 39 kDa. The expected band size for c-Jun/JUN is at 33 kDa.IF
IF analysis of c-Jun/JUN using anti-c-Jun/JUN antibody .c-Jun/JUN was detected in a paraffin-embedded section of zebrafish embryo tissue. The tissue section was incubated with rabbit anti-c-Jun/JUN Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody. The section was counterstained with DAPI .| Product Name | Anti-Zebrafish c-Jun/JUN Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived Zebrafish c-Jun/JUN recombinant protein (Position: M1-F308). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIF |
| WB:1:500-1:2000 IF:1:50-1:400 |
|
| Species Reactivity | Zebrafish |
| Concentration | 500ug/ml |
| Purification | Affinity purified |
| Gene Full Name | Jun proto-oncogene, AP-1 transcription factor subunit |
|---|---|
| Target | JUN |
| Alternative Names | Activator protein 1 AP 1 AP1 c Jun JUN jun oncogene P39 Proto oncogene c Jun Transcription factor AP 1 |
| Molecular Weight(MW) | 33 kDa(Observed band size: 39 kDa) |
| SwissProt ID | Q6NZT5 |
|---|
WB
Western blot analysis of c-Jun/JUN using anti-c-Jun/JUN antibody . The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: zebrafish head tissue lysates,Lane 2: whole female zebrafish tissue lysates,Lane 3: whole male zebrafish tissue lysates,Lane 4: zebrafish embryo tissue lysates.After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-c-Jun/JUN antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using ECL Plus Western Blotting Substrate . A specific band was detected for c-Jun/JUN at approximately 39 kDa. The expected band size for c-Jun/JUN is at 33 kDa.
IF
IF analysis of c-Jun/JUN using anti-c-Jun/JUN antibody .c-Jun/JUN was detected in a paraffin-embedded section of zebrafish embryo tissue. The tissue section was incubated with rabbit anti-c-Jun/JUN Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody. The section was counterstained with DAPI .| Application Notes | WB:1:500-1:2000 IF:1:50-1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt,-20℃ as supplied. |
| Storage Buffer | PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
