WB
Western blot analysis of GATA4 using anti-GATA4 antibody . The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: zebrafish head tissue lysates,Lane 2: whole female zebrafish tissue lysates,Lane 3: whole male zebrafish tissue lysates,Lane 4: zebrafish embryo tissue lysates.After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GATA4 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using ECL Plus Western Blotting Substrate . A specific band was detected for GATA4 at approximately 42 kDa. The expected band size for GATA4 is at 37 kDa.| Product Name | Anti-Zebrafish GATA4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived Zebrafish GATA4 recombinant protein (Position: M1-M332) |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-1:2000 |
|
| Species Reactivity | Zebrafish |
| Concentration | 500ug/ml |
| Purification | Affinity purified |
| Gene Full Name | GATA binding protein 4 |
|---|---|
| Target | GATA4 |
| Alternative Names | GATA binding factor 4 GATA binding protein 4 GATA4 Transcription factor GATA 4 |
| Molecular Weight(MW) | 37 kDa(Observed band size: 42 kDa) |
| SwissProt ID | Q09JY7 |
|---|
WB
Western blot analysis of GATA4 using anti-GATA4 antibody . The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: zebrafish head tissue lysates,Lane 2: whole female zebrafish tissue lysates,Lane 3: whole male zebrafish tissue lysates,Lane 4: zebrafish embryo tissue lysates.After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GATA4 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using ECL Plus Western Blotting Substrate . A specific band was detected for GATA4 at approximately 42 kDa. The expected band size for GATA4 is at 37 kDa.| Application Notes | WB:1:500-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt,-20℃ as supplied. |
| Storage Buffer | PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
