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WB
Western blot analysis of Nestin in mouse brain , rat brain lysates and using Nestin Goat pAb.IF
Immunofluorescence analysis of Nestin(Green) in mouse brain tissue using Nestin Goat pAb, and DAPI(Blue)IHC
Immunohistochemistry of Paraffin-embedded human breast tissue sections using the antibody Nestin Goat pAb at a dilution of 1/50. Heat-induced epitope retrieval was performed using sodium citrate buffer (pH 6.0).| Product Name | Nestin Goat pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant Human Nestin |
| Modification | Unmodified |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Goat |
| Tested Applications | IHCWBIF |
| WB:1:500-1:1000 IHC:1:100-1:500 IF-P:1:100-1:500 ICC/IF:1:100-1:300 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity Purified |
| Gene Symbol | NES |
|---|---|
| Gene Synonyms | Nbla00170 |
| Gene Full Name | nestin |
| Gene Summary | This gene encodes a member of the intermediate filament protein family and is expressed primarily in nerve cells. [provided by RefSeq, Sep 2011] |
| Molecular Weight(MW) | 180 kDa |
| Function | Nestin is a Class VI intermediate filament abundantly produced in the developing central nervous system (CNS) and in early embryonic neuroepithelial stem cells. Nestin has been widely used as a predominant marker for stem/progenitor cells, glioma cells, and tumor endothelial cells. Also, Nestin is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumors. Highly specific antibodies to human Nestin can aid in characterizing progenitor cells differentiating into distinct lineages, in enhancing the therapeutic potential of human neural stem/progenitor cells in the treatment of CNS diseases or injury, and in identifying neuroepithelial tumor cells. Nestin expression has been observed in pancreatic hematopoietic stem cell populations, and is considered a novel angiogenesis marker of proliferating endothelial cells in colorectal cancer tissue. |
| Cellular Localization | Intermediate filament |
WB
Western blot analysis of Nestin in mouse brain , rat brain lysates and using Nestin Goat pAb.
IF
Immunofluorescence analysis of Nestin(Green) in mouse brain tissue using Nestin Goat pAb, and DAPI(Blue)
IHC
Immunohistochemistry of Paraffin-embedded human breast tissue sections using the antibody Nestin Goat pAb at a dilution of 1/50. Heat-induced epitope retrieval was performed using sodium citrate buffer (pH 6.0).| Application Notes | WB:1:500-1:1000 IHC:1:100-1:500 IF-P:1:100-1:500 ICC/IF:1:100-1:300 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Storage Buffer | Liquid in PBS, 50% glycerol, 0.05% Proclin 300, 0.08%BSA |
