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WB
Western blot analysis of SOX9 in mouse brain , rat brain lysates and using SOX9 Goat pAb.IF
Immunofluorescence analysis of SOX9 (Green) in mouse brain tissue using SOX9 Goat pAb, and DAPI(Blue)IHC
Immunohistochemistry of Paraffin-embedded human pancreas tissue sections using the antibody SOX9 Goat pAb at a dilution of 1/50. Heat-induced epitope retrieval was performed using Tris-EDTA（pH9.0）.IHC
Immunohistochemistry of Paraffin-embedded human tonsil using the antibody SOX9 Goat pAb at a dilution of 1/50. Heat-induced epitope retrieval was performed using Tris-EDTA（pH9.0）.| Product Name | SOX9 Goat pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant Human SOX9 |
| Modification | Unmodified |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Goat |
| Tested Applications | IHCWBIF |
| WB:1:500-1:1000 IHC:1:100-1:500 IF-P:1:100-1:500 ICC/IF:1:100-1:300 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity Purified |
| Gene Symbol | SOX9 |
|---|---|
| Gene Synonyms | CMD1 SRA1 CMPD1 ENH13 SRXX2 TESCO SRXY10 |
| Gene Full Name | SRY-box transcription factor 9 |
| Gene Summary | The protein encoded by this gene recognizes the sequence CCTTGAG along with other members of the HMG-box class DNA-binding proteins. It acts during chondrocyte differentiation and, with steroidogenic factor 1, regulates transcription of the anti-Muellerian hormone (AMH) gene. Deficiencies lead to the skeletal malformation syndrome campomelic dysplasia, frequently with sex reversal. [provided by RefSeq, Jul 2008] |
| Molecular Weight(MW) | 56 kDa |
| Function | Regulates several important processes during embryonic development including chondrogenesis, during which it contributes to skeletal formation and digit specification (2,3). Sox9 also coordinates with steroidogenic factor-1 to direct Sertoli cell-specific expression of anti-Mullerian hormone during embryogenesis, thereby contributing to male sex determination. |
| Cellular Localization | Nucleus |
WB
Western blot analysis of SOX9 in mouse brain , rat brain lysates and using SOX9 Goat pAb.
IF
Immunofluorescence analysis of SOX9 (Green) in mouse brain tissue using SOX9 Goat pAb, and DAPI(Blue)
IHC
Immunohistochemistry of Paraffin-embedded human pancreas tissue sections using the antibody SOX9 Goat pAb at a dilution of 1/50. Heat-induced epitope retrieval was performed using Tris-EDTA（pH9.0）.
IHC
Immunohistochemistry of Paraffin-embedded human tonsil using the antibody SOX9 Goat pAb at a dilution of 1/50. Heat-induced epitope retrieval was performed using Tris-EDTA（pH9.0）.| Application Notes | WB:1:500-1:1000 IHC:1:100-1:500 IF-P:1:100-1:500 ICC/IF:1:100-1:300 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Storage Buffer | Liquid in PBS, 50% glycerol, 0.05% Proclin 300, 0.08%BSA |
