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WB
Western blot analysis of Doublecortin in mouse brain , rat brain lysates and using Doublecortin Goat pAb.IF
Immunofluorescence analysis of Doublecortin (Green) in mouse brain tissue using Doublecortin Goat pAb, and DAPI(Blue)IHC
Immunohistochemistry of Paraffin-embedded mouse testis tissue sections using the antibody Doublecortin Goat pAb at a dilution of 1/50. Heat-induced epitope retrieval was performed using Tris-EDTA（pH9.0）.| Product Name | Doublecortin Goat pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant Human Doublecortin |
| Modification | Unmodified |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Goat |
| Tested Applications | IHCWBIF |
| WB:1:500-1:1000 IHC:1:100-1:500 IF-P:1:100-1:500 ICC/IF:1:100-1:300 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity Purified |
| Gene Symbol | DCX |
|---|---|
| Gene Synonyms | DC DBCN LISX SCLH XLIS |
| Gene Full Name | doublecortin |
| Gene Summary | This gene encodes a member of the doublecortin family. The protein encoded by this gene is a cytoplasmic protein and contains two doublecortin domains, which bind microtubules. In the developing cortex, cortical neurons must migrate over long distances to reach the site of their final differentiation. The encoded protein appears to direct neuronal migration by regulating the organization and stability of microtubules. In addition, the encoded protein interacts with LIS1, the regulatory gamma subunit of platelet activating factor acetylhydrolase, and this interaction is important to proper microtubule function in the developing cortex. Mutations in this gene cause abnormal migration of neurons during development and disrupt the layering of the cortex, leading to epilepsy, cognitive disability, subcortical band heterotopia ("double cortex" syndrome) in females and lissencephaly ("smooth brain" syndrome) in males. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2010] |
| Molecular Weight(MW) | 45 kDa |
| Function | Mutations in Doublecortin cause Lissencephaly (smooth brain), a neuronal migration disorder characterized by epilepsy and mental retardation. Doublecortin is a microtubule associated protein that stabilizes and bundles microtubules. A conserved doublecortin domain mediates the interaction with microtubules, and interestingly most missense mutations cluster in this domain. |
| Cellular Localization | Cell projection,Cytoplasm,Microtubule |
WB
Western blot analysis of Doublecortin in mouse brain , rat brain lysates and using Doublecortin Goat pAb.
IF
Immunofluorescence analysis of Doublecortin (Green) in mouse brain tissue using Doublecortin Goat pAb, and DAPI(Blue)
IHC
Immunohistochemistry of Paraffin-embedded mouse testis tissue sections using the antibody Doublecortin Goat pAb at a dilution of 1/50. Heat-induced epitope retrieval was performed using Tris-EDTA（pH9.0）.| Application Notes | WB:1:500-1:1000 IHC:1:100-1:500 IF-P:1:100-1:500 ICC/IF:1:100-1:300 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Storage Buffer | Liquid in PBS, 50% glycerol, 0.05% Proclin 300, 0.08%BSA |
