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WB
Western blot analysis of Connexin 43 in Hela,mouse brain and rat brain lysates using Connexin 43 Goat pAb.IF
Immunofluorescence analysis of Connexin 43 (Green) in mouse parathyroid glandIHC
Immunohistochemistry of Paraffin-embedded human thyroid cancer tissue sections using the antibody Connexin 43 Goat pAb at a dilution of 1/50. Heat-induced epitope retrieval was performed using sodium citrate buffer (pH 6.0).| Product Name | Connexin 43 / GJA1 Goat pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant Human Connexin 43 |
| Modification | Unmodified |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Goat |
| Tested Applications | IHCWBIF |
| WB:1:500-1:1000 IHC:1:100-1:500 IF-P:1:100-1:500 ICC/IF:1:100-1:300 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity Purified |
| Gene Symbol | GJA1 |
|---|---|
| Gene Synonyms | HSS CMDR CX43 EKVP GJAL ODDD AVSD3 EKVP3 HLHS1 PPKCA |
| Gene Full Name | gap junction protein alpha 1 |
| Gene Summary | This gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. The encoded protein is the major protein of gap junctions in the heart that are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. A related intronless pseudogene has been mapped to chromosome 5. Mutations in this gene have been associated with oculodentodigital dysplasia, autosomal recessive craniometaphyseal dysplasia and heart malformations. [provided by RefSeq, May 2014] |
| Molecular Weight(MW) | 43 kDa |
| Function | Connexin-43 (Cx43, also known as gap junction alpha-1), a member of connexin family, plays essential roles in gap junction communication that facilitates direct communication among adjacent cells (PMID: 12270943). Usually, six connexin proteins oligomerize into a hemi-channel or connexon during intercellular channel formation (PMID: 12270943). Mutations of Cx43 may cause a series of diseases such as oculodentodigital dysplasia (ODDD), syndactyly 3 (SDTY3), hypoplastic left heart syndrome 1 (HLHS1) and so on (PMID: 18161618, 1472936, 1147490). |
| Cellular Localization | Cell junction,Cell membrane,Endoplasmic reticulum,Gap junction,Membrane |
WB
Western blot analysis of Connexin 43 in Hela,mouse brain and rat brain lysates using Connexin 43 Goat pAb.
IF
Immunofluorescence analysis of Connexin 43 (Green) in mouse parathyroid gland
IHC
Immunohistochemistry of Paraffin-embedded human thyroid cancer tissue sections using the antibody Connexin 43 Goat pAb at a dilution of 1/50. Heat-induced epitope retrieval was performed using sodium citrate buffer (pH 6.0).| Application Notes | WB:1:500-1:1000 IHC:1:100-1:500 IF-P:1:100-1:500 ICC/IF:1:100-1:300 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Storage Buffer | Liquid in PBS, 50% glycerol, 0.05% Proclin 300, 0.08%BSA |
