WB
Western blot analysis of CK5 in Hela , NIH/3T3 lysates and using CK5 Goat pAb.IF
Immunofluorescence analysis of CK5(Green) in Human skin tissue using CK5 Goat pAb, and DAPI(Blue)| Product Name | Cytokeratin 5 Goat pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant Human CK5 |
| Modification | Unmodified |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Goat |
| Tested Applications | WBIF |
| WB:1:500-1:1000 IHC:1:100-1:500 IF-P:1:100-1:500 ICC/IF:1:100-1:300 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity Purified |
| Gene Symbol | KRT5 |
|---|---|
| Gene Synonyms | K5 CK5 DDD DDD1 EBS1 EBS2 EBS2A EBS2B EBS2C EBS2D EBS2E EBS2F KRT5A |
| Gene Full Name | keratin 5 |
| Gene Summary | The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq, Jul 2008] |
| Molecular Weight(MW) | 62 kDa |
| Function | The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. |
| Cellular Localization | Cytoplasm,Intermediate filament,Keratin |
WB
Western blot analysis of CK5 in Hela , NIH/3T3 lysates and using CK5 Goat pAb.
IF
Immunofluorescence analysis of CK5(Green) in Human skin tissue using CK5 Goat pAb, and DAPI(Blue)| Application Notes | WB:1:500-1:1000 IHC:1:100-1:500 IF-P:1:100-1:500 ICC/IF:1:100-1:300 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Storage Buffer | Liquid in PBS, 50% glycerol, 0.05% Proclin 300, 0.08%BSA |
