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WB
Western blot analysis of Tubulin beta 3 in MCF-7,mouse brain and rat brain lysates using Tubulin beta 3 Goat pAb.IF
Immunofluorescence analysis of Tubulin beta 3(Green) in mouse brain tissue using Tubulin beta 3 Goat pAb, and DAPI(Blue)IHC
Immunohistochemistry of Paraffin-embedded mouse brain tissue sections using the antibody Tubulin beta 3 Goat pAb at a dilution of 1/50. Heat-induced epitope retrieval was performed using Tris-EDTA（pH9.0）.| Product Name | beta III Tubulin Goat pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant Human Tubulin beta 3 |
| Modification | Unmodified |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Goat |
| Tested Applications | IHCWBIF |
| WB:1:500-1:1000 IHC:1:100-1:500 IF-P:1:100-1:500 ICC/IF:1:100-1:300 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity Purified |
| Gene Symbol | TUBB3 |
|---|---|
| Gene Synonyms | CDCBM FEOM3 TUBB4 CDCBM1 CFEOM3 beta-4 CFEOM3A |
| Gene Full Name | tubulin beta 3 class III |
| Gene Summary | This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6. [provided by RefSeq, Oct 2010] |
| Molecular Weight(MW) | 55 kDa(Observed band size: 50 kDa) |
| Function | TUBB3 Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. TUBB3 plays a critical role in proper axon guidance and mantainance. |
| Cellular Localization | Cell projection,Cytoplasm,Cytoskeleton,Microtubule |
WB
Western blot analysis of Tubulin beta 3 in MCF-7,mouse brain and rat brain lysates using Tubulin beta 3 Goat pAb.
IF
Immunofluorescence analysis of Tubulin beta 3(Green) in mouse brain tissue using Tubulin beta 3 Goat pAb, and DAPI(Blue)
IHC
Immunohistochemistry of Paraffin-embedded mouse brain tissue sections using the antibody Tubulin beta 3 Goat pAb at a dilution of 1/50. Heat-induced epitope retrieval was performed using Tris-EDTA（pH9.0）.| Application Notes | WB:1:500-1:1000 IHC:1:100-1:500 IF-P:1:100-1:500 ICC/IF:1:100-1:300 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Storage Buffer | Liquid in PBS, 50% glycerol, 0.05% Proclin 300, 0.08%BSA |
