WB
Western blot analysis of OPN in mouse brain , rat brain lysates and using OPN Goat pAb.IF
Immunofluorescence analysis of OPN(Green) in mouse colon tissue using OPN Goat pAb, and DAPI(Blue)| Product Name | Osteopontin Goat pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant Human OPN |
| Modification | Unmodified |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Goat |
| Tested Applications | WBIF |
| WB:1:500-1:1000 IHC:1:100-1:500 IF-P:1:100-1:500 ICC/IF:1:100-1:300 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity Purified |
| Gene Symbol | SPP1 |
|---|---|
| Gene Synonyms | OPN BNSP BSPI ETA-1 |
| Gene Full Name | secreted phosphoprotein 1 |
| Gene Summary | The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011] |
| Molecular Weight(MW) | 58-75 kDa(Observed band size: 70 kDa) |
| Function | Major non-collagenous bone protein that binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity. |
| Cellular Localization | Secreted |
WB
Western blot analysis of OPN in mouse brain , rat brain lysates and using OPN Goat pAb.
IF
Immunofluorescence analysis of OPN(Green) in mouse colon tissue using OPN Goat pAb, and DAPI(Blue)| Application Notes | WB:1:500-1:1000 IHC:1:100-1:500 IF-P:1:100-1:500 ICC/IF:1:100-1:300 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Storage Buffer | Liquid in PBS, 50% glycerol, 0.05% Proclin 300, 0.08%BSA |
