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WB
Western blot analysis of Vimentin in mouse brain , rat brain lysates and using Vimentin Goat pAb.IF
Immunofluorescence analysis of Vimentin (Green) in human Breast cancer tissue using Vimentin Goat pAb, and DAPI(Blue)IHC
Immunohistochemistry of Paraffin-embedded human tonsil tissue sections using the antibody Vimentin Goat pAb at a dilution of 1/50. Heat-induced epitope retrieval was performed using Tris-EDTA（pH9.0）.| Product Name | Vimentin Goat pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant Human vim |
| Modification | Unmodified |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Goat |
| Tested Applications | IHCWBIF |
| WB:1:500-1:1000 IHC:1:100-1:500 IF-P:1:100-1:500 ICC/IF:1:100-1:300 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity Purified |
| Gene Symbol | VIM |
|---|---|
| Gene Full Name | vimentin |
| Gene Summary | This gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients. [provided by RefSeq, Aug 2017] |
| Molecular Weight(MW) | 53 kDa |
| Function | Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Unlike other IFP proteins, vimentin is expressed, along with desmin, during the early stages of cellular development. During the development process, vimentin is exchanged for new, tissue-specific IFPs. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis. |
| Cellular Localization | Cell membrane,Cell projection,Cytoplasm,Cytoskeleton,Intermediate filament,Membrane,Nucleus |
WB
Western blot analysis of Vimentin in mouse brain , rat brain lysates and using Vimentin Goat pAb.
IF
Immunofluorescence analysis of Vimentin (Green) in human Breast cancer tissue using Vimentin Goat pAb, and DAPI(Blue)
IHC
Immunohistochemistry of Paraffin-embedded human tonsil tissue sections using the antibody Vimentin Goat pAb at a dilution of 1/50. Heat-induced epitope retrieval was performed using Tris-EDTA（pH9.0）.| Application Notes | WB:1:500-1:1000 IHC:1:100-1:500 IF-P:1:100-1:500 ICC/IF:1:100-1:300 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Storage Buffer | Liquid in PBS, 50% glycerol, 0.05% Proclin 300, 0.08%BSA |
