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WB
Western blot analysis of IBA1 in mouse brain , rat brain lysates and using IBA1 Goat pAb.IF
Immunofluorescence analysis of IBA1(Green) in mouse brain tissue using IBA1 Goat pAb, and DAPI(Blue)IHC
Immunohistochemistry of Paraffin-embedded mouse brain tissue sections using the antibody IBA1 Goat pAb at a dilution of 1/50. Heat-induced epitope retrieval was performed using Tris-EDTA（pH9.0）.| Product Name | Iba1 Goat pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant Human IBA1 |
| Modification | Unmodified |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Goat |
| Tested Applications | IHCWBIF |
| WB:1:500-1:1000 IHC:1:100-1:500 IF-P:1:100-1:500 ICC/IF:1:100-1:300 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity Purified |
| Gene Symbol | AIF1 |
|---|---|
| Gene Synonyms | IBA1 IRT1 AIF-1 IRT-1 |
| Gene Full Name | allograft inflammatory factor 1 |
| Gene Summary | This gene encodes a protein that binds actin and calcium. This gene is induced by cytokines and interferon and may promote macrophage activation and growth of vascular smooth muscle cells and T-lymphocytes. Polymorphisms in this gene may be associated with systemic sclerosis. Alternative splicing results in multiple transcript variants, but the full-length and coding nature of some of these variants is not certain. [provided by RefSeq, Jan 2016] |
| Molecular Weight(MW) | 17 kDa |
| Function | Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. |
| Cellular Localization | Cell membrane,Cell projection,Cytoplasm,Cytoskeleton,Membrane |
WB
Western blot analysis of IBA1 in mouse brain , rat brain lysates and using IBA1 Goat pAb.
IF
Immunofluorescence analysis of IBA1(Green) in mouse brain tissue using IBA1 Goat pAb, and DAPI(Blue)
IHC
Immunohistochemistry of Paraffin-embedded mouse brain tissue sections using the antibody IBA1 Goat pAb at a dilution of 1/50. Heat-induced epitope retrieval was performed using Tris-EDTA（pH9.0）.| Application Notes | WB:1:500-1:1000 IHC:1:100-1:500 IF-P:1:100-1:500 ICC/IF:1:100-1:300 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Storage Buffer | Liquid in PBS, 50% glycerol, 0.05% Proclin 300, 0.08%BSA |
