WB
Western blot analysis of NeuN in mouse brain , rat brain lysates and using NeuN Goat pAb.IF
Immunofluorescence analysis of NeuN(Green) in mouse brain tissue using NeuN Goat pAb, and DAPI(Blue)| Product Name | NeuN Goat pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant Human NeuN |
| Modification | Unmodified |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Goat |
| Tested Applications | WBIF |
| WB:1:500-1:1000 IHC:1:100-1:500 IF-P:1:100-1:500 ICC/IF:1:100-1:300 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity Purified |
| Gene Symbol | RBFOX3 |
|---|---|
| Gene Synonyms | FOX3 NEUN FOX-3 HRNBP3 |
| Gene Full Name | RNA binding fox-1 homolog 3 |
| Gene Summary | This gene encodes a member of the RNA-binding FOX protein family which is involved in the regulation of alternative splicing of pre-mRNA. The protein has an N-terminal proline-rich region, an RNA recognition motif (RRM) domain, and a C-terminal alanine-rich region. This gene produces the neuronal nuclei (NeuN) antigen that has been widely used as a marker for post-mitotic neurons. This gene has its highest expression in the central nervous system and plays a prominent role in neural tissue development and regulation of adult brain function. Mutations in this gene have been associated with numerous neurological disorders. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, May 2017] |
| Molecular Weight(MW) | 50 kDa(Observed band size: 55 kDa) |
| Function | NeuN, also named as FOX3 and RBFOX3, is neuronal-specific nuclear protein with MW 38-50kd(~48kd). It is one of a family of 3 mammalian Fox homologues. FOX3(Or NeuN) functions in RNA-binding protein that regulates alternative splicing events. |
| Cellular Localization | Cytoplasm,Nucleus |
WB
Western blot analysis of NeuN in mouse brain , rat brain lysates and using NeuN Goat pAb.
IF
Immunofluorescence analysis of NeuN(Green) in mouse brain tissue using NeuN Goat pAb, and DAPI(Blue)| Application Notes | WB:1:500-1:1000 IHC:1:100-1:500 IF-P:1:100-1:500 ICC/IF:1:100-1:300 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Storage Buffer | Liquid in PBS, 50% glycerol, 0.05% Proclin 300, 0.08%BSA |
