ICC/IF
AF488 staining on IF;Sample: HepG2 cellPrimary Ab: 30µg/ml Mouse Anti-Human Trx AntibodySecond Ab: 2μg/ml AF488-Linked Caprine Anti-Mouse IgG Polyclonal AntibodyFC
Human A549 cell line was fixed with 2% paraformaldehyde (10 min), permeabilised with 0.1% BSA-Triton X-100,then stained with 10µg/ml mouse Anti-human Trx Monoclonal Antibody (red histogram) or Isotype control antibody (green histogram), followed by 1µg/ml FITC-conjugated Anti-mouse IgG Secondary Antibody.| Product Name | Monoclonal Antibody to Thioredoxin (Trx) |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | RPA702Hu01-Recombinant Thioredoxin (Trx) |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 Kappa |
| Host Species | Mouse |
| Tested Applications | ICC/IFFCM |
| ICC/IF: 5-20µg/mL Flow cytometry: 10µg/mL |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A/G |
| Gene Symbol | trx |
|---|---|
| Gene Synonyms | 4720,4733,CG8651,Dmel\CG8651,DMTRXIII,l(3)bx[v],l(3)j14A6,l(3)j3A2,l(3)s5452,NR0A5,R-bx,Rg-bx,tqrx,Trx,TrX,TRX,Trx-C,trx-g,Trx-G,Trx/MLL,trxG,TrxG,trxI and trxII |
| Gene Full Name | trithorax |
| Gene Summary | Enables several functions, including protein homodimerization activity,protein methyltransferase activity,and protein phosphatase 1 binding activity. Involved in several processes, including germ cell migration,haltere development,and positive regulation of transcription elongation by RNA polymerase II. Located in nucleus and polytene chromosome band. Part of MLL1/2 complex,histone acetyltransferase complex,and transcription elongation factor complex. Is expressed in several structures, including epithelium,ganglia,gut section,head epidermis primordium,and imaginal disc. Used to study congenital heart disease. Human ortholog(s) of this gene implicated in autosomal dominant intellectual developmental disorder 68,cervical cancer,dystonia 28, childhood-onset,gastrointestinal system cancer (multiple),and hematologic cancer (multiple). Orthologous to human KMT2A (lysine methyltransferase 2A) and KMT2B (lysine methyltransferase 2B). [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | TXN ADF SASP TRDX TRX1 ATL-derived factor Surface-associated sulphydryl protein |
ICC/IF
AF488 staining on IF;Sample: HepG2 cellPrimary Ab: 30µg/ml Mouse Anti-Human Trx AntibodySecond Ab: 2μg/ml AF488-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
FC
Human A549 cell line was fixed with 2% paraformaldehyde (10 min), permeabilised with 0.1% BSA-Triton X-100,then stained with 10µg/ml mouse Anti-human Trx Monoclonal Antibody (red histogram) or Isotype control antibody (green histogram), followed by 1µg/ml FITC-conjugated Anti-mouse IgG Secondary Antibody.| Application Notes | ICC/IF: 5-20µg/mL Flow cytometry: 10µg/mL |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles. |
| Storage Buffer | 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol. |
