ELISA
Coat the plate with immunizing antigen.Blue line： Antibody; Orange line: Isotype control antibodyWB
Western blot analysis using PAK2 antibody against K562(1),A549(2),Cos-7(3),C2C12(4),Hek293(5) cell,Rat liver tissue(6),Rat stomach tissue(7) . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:2000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.| Product Name | PAK2 Rabbit mAb,pbs only |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| WB:1:1000-1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | PAK2 |
|---|---|
| Gene Synonyms | 5330420P17Rik A130002K10Rik alpha-PAK D16Ertd269e gamma-PAK IDDMSSD KNO2 mKIAA4182 p65-PAK PAK-1 PAK-2 PAK65 Paka PAKalpha PAKgamma |
| Gene Full Name | p21 (RAC1) activated kinase 2 |
| Gene Summary | The p21 activated kinases (PAK) are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. The PAK proteins are a family of serine/threonine kinases that serve as targets for the small GTP binding proteins, CDC42 and RAC1, and have been implicated in a wide range of biological activities. The protein encoded by this gene is activated by proteolytic cleavage during caspase-mediated apoptosis, and may play a role in regulating the apoptotic events in the dying cell. [provided by RefSeq, Jul 2008] |
| Alternative Names | activated protein kinase alpha alpha-PAK Beta-PAK CDC42/RAC effector kinase PAK-A gamma pak gamma-PAK Oligophrenin-3 OPHN3 p21 (CDKN1A)-activated kinase 1 p21 (CDKN1A)-activated kinase 2 P21 CDKN1A-activated kinase 1 p21 protein (Cdc42/Rac)-activated kinase 1 p21 protein (Cdc42/Rac)-activated kinase 2 p21-activated kinase 1 p21-activated kinase 2 p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog yeast) p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related) p21cdc42/rac1-activated serine/threonine kinase p58 p65-PAK p68-PAK PAK alpha PAK beta PAK gamma PAK-1 PAK-2 PAK-3 PAK3 protein kinase protein kinase MUK2 S6/H4 kinase serine/threonine-protein kinase PAK 2-like STE20 homolog yeast |
| Molecular Weight(MW) | 58kDa |
| Cellular Localization | Cytoplasm, Membrane, Nucleus |
| SwissProt ID | Q13177 |
|---|
ELISA
Coat the plate with immunizing antigen.Blue line： Antibody; Orange line: Isotype control antibody
WB
Western blot analysis using PAK2 antibody against K562(1),A549(2),Cos-7(3),C2C12(4),Hek293(5) cell,Rat liver tissue(6),Rat stomach tissue(7) . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:2000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.| Application Notes | WB:1:1000-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | PBS ONLY |
