ELISA
Coat the plate with immunizing antigen.Blue line： Antibody; Orange line: Isotype control antibodyWB
Western blot analysis using ABL1 (phospho Y412) antibody against Hela(1) ,K562(2) ,A549(3) ,Huvec(4) ,Nih3t3(5)cell . 10% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:2000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.| Product Name | ABL1 (phospho Y412) Rabbit mAb,pbs only |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| WB:1:1000-1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | ABL1 |
|---|---|
| Gene Synonyms | ABL BCR-ABL bcr/abl c-ABL c-ABL1 CHDSKM E430008G22Rik JTK7 p150 v-abl |
| Gene Full Name | ABL proto-oncogene 1, non-receptor tyrosine kinase |
| Gene Summary | This gene is a protooncogene that encodes a protein tyrosine kinase involved in a variety of cellular processes, including cell division, adhesion, differentiation, and response to stress. The activity of the protein is negatively regulated by its SH3 domain, whereby deletion of the region encoding this domain results in an oncogene. The ubiquitously expressed protein has DNA-binding activity that is regulated by CDC2-mediated phosphorylation, suggesting a cell cycle function. This gene has been found fused to a variety of translocation partner genes in various leukemias, most notably the t(9;22) translocation that results in a fusion with the 5' end of the breakpoint cluster region gene (BCR; MIM:151410). Alternative splicing of this gene results in two transcript variants, which contain alternative first exons that are spliced to the remaining common exons. [provided by RefSeq, Aug 2014] |
| Alternative Names | Abelson murine leukemia oncogene abelson murine leukemia viral oncogene homolog 1 Abelson tyrosine-protein kinase 1 Abl 1 antib ABL kinase domain abl protein ABL protooncogene 1 nonreceptor tyrosine kinase ABL1 ABL2 ABLL ARG B30 BCR-ABL1 p190 BCR/ABL e8a2 fusion BCR/ABL variant p190 fusion protein BCR/ABL variant-p210 fusion protein BCR/ABL1 e1a2 fusion protein BCR/ABL1 fusion BCR/ABL1 fusion protein e3a1 bcr/c-abl oncogene protein BCR::ABL1 fusion protein c-abl oncogene 1 non-receptor tyrosine kinase c-abl oncogene 1 receptor tyrosine kinase c-abl protein type I c-abl protein type II c-abl protein type III c-abl-protein type I c-abl-protein type II c-abl-protein type III c-abl-protein type IV chimeric BCR::ABL1 protein H1681-BCR/ABL variant p210 fusion protein non-receptor tyrosine kinase p150 p190 proto-oncogene c-Abl proto-oncogene tyrosine-protein kinase ABL1 RP11-83J21.1 Tyrosine kinase ARG tyrosine kinase protein v-abl Abelson murine leukemia oncogene 1 v-abl Abelson murine leukemia viral oncogene homolog 1 |
| Molecular Weight(MW) | 128kDa |
| Cellular Localization | Cytoplasm, cytoskeleton, Nucleus, Mitochondrion; Nucleus membrane |
| SwissProt ID | P00519 |
|---|
ELISA
Coat the plate with immunizing antigen.Blue line： Antibody; Orange line: Isotype control antibody
WB
Western blot analysis using ABL1 (phospho Y412) antibody against Hela(1) ,K562(2) ,A549(3) ,Huvec(4) ,Nih3t3(5)cell . 10% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:2000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.| Application Notes | WB:1:1000-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | PBS ONLY |
