ELISA
Coat the plate with immunizing antigen.Blue line： Antibody; Orange line: Isotype control antibodyWB
Western blot analysis using HIF-2 alpha antibody against Hela(1),Hepg2(2),K562(3),A549(4),A431(5),Huvec(6)cell . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:2000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.| Product Name | HIF-2 alpha Rabbit mAb,pbs only |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| WB:1:1000-1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | EPAS1 |
|---|---|
| Gene Synonyms | bHLHe73 ECYT4 HIF-2 alpha HIF-2alpha HIF2 alpha HIF2A HLF HRF MOP2 PASD2 |
| Gene Full Name | endothelial PAS domain protein 1 |
| Gene Summary | This gene encodes a transcription factor involved in the induction of genes regulated by oxygen, which is induced as oxygen levels fall. The encoded protein contains a basic-helix-loop-helix domain protein dimerization domain as well as a domain found in proteins in signal transduction pathways which respond to oxygen levels. Mutations in this gene are associated with erythrocytosis familial type 4. [provided by RefSeq, Nov 2009] |
| Alternative Names | basic-helix-loop-helix-PAS protein MOP2 class E basic helix-loop-helix protein 73 EPAS-1 Hif like protein HIF-1-alpha-like factor HIF-1alpha-like factor HIF-2-alpha HIF-related factor HIF1 alpha-like factor HIF1alpha-like factor HIF2-alpha HLF (HIF1alpha-like factor) HRF hypoxia inducible factor 2 alpha subunit hypoxia inducible factor 2a hypoxia inducible transcription factor 2alpha hypoxia-inducible factor 2 alpha hypoxia-inducible factor 2-alpha member of PAS protein 2 mHLF PAS domain-containing protein 2 |
| Molecular Weight(MW) | 96kDa |
| Cellular Localization | Nucleus, Nucleus speckle |
| SwissProt ID | Q99814 |
|---|
ELISA
Coat the plate with immunizing antigen.Blue line： Antibody; Orange line: Isotype control antibody
WB
Western blot analysis using HIF-2 alpha antibody against Hela(1),Hepg2(2),K562(3),A549(4),A431(5),Huvec(6)cell . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:2000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.| Application Notes | WB:1:1000-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | PBS ONLY |
