ELISA
Coat the plate with immunizing antigen.Blue line： Antibody; Orange line: Isotype control antibodyWB
Western blot analysis using SLC40A1 antibody against Hepg2(1),K562(2),Mcf-7(3) cell . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:2000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.| Product Name | SLC40A1 Rabbit mAb,pbs only |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| WB:1:1000-1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | SLC40A1 |
|---|---|
| Gene Synonyms | Dusg FPN FPN1 HFE4 IREG1 MST079 MSTP079 MTP MTP1 Ol5 Pcm SLC11A3 Slc39a1 |
| Gene Full Name | solute carrier family 40 member 1 |
| Gene Summary | The protein encoded by this gene is a cell membrane protein that may be involved in iron export from duodenal epithelial cells. Defects in this gene are a cause of hemochromatosis type 4 (HFE4). [provided by RefSeq, Jul 2008] |
| Alternative Names | CAR1 cell adhesion regulator duodenal-specific ferroportin 1 ferroportin 1 ireg1 ferroportin-1 ferroportin1 FPN-N iron regulated gene 1 iron-regulated transporter 1 metal transporter protein 1 metal transporting protein 1 polycythaemia putative cellular iron exporter SLC11A3 iron transporter SLC40 iron transporter SLC40A1 solute carrier family 11 (proton-coupled divalent metal ion transporters) member 3 solute carrier family 39 (iron-regulated transporter) member 1 solute carrier family 40 (iron-regulated transporter) member 1 solute carrier family 40 member 1 transporter transporter Fpn1 |
| Molecular Weight(MW) | 63kDa |
| Cellular Localization | Cell membrane, Basolateral cell membrane |
| SwissProt ID | Q9NP59 |
|---|
ELISA
Coat the plate with immunizing antigen.Blue line： Antibody; Orange line: Isotype control antibody
WB
Western blot analysis using SLC40A1 antibody against Hepg2(1),K562(2),Mcf-7(3) cell . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:2000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.| Application Notes | WB:1:1000-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | PBS ONLY |
