ELISA
Coat the plate with immunizing antigen.Blue line： Antibody; Orange line: Isotype control antibodyWB
Western blot analysis using HSPA1L antibody against Hela(1),Hepg2(2),K562(3),A549(4) cell,Mouse lung tissue(5),Mouse spleen tissue(6),Mouse stomach tissue(7),Mouse heart tissue(8) . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:2000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.| Product Name | HSPA1L Rabbit mAb,pbs only |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| WB:1:1000-1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Antigen peptide affinity chromatography |
| Gene Symbol | HSPA1L |
|---|---|
| Gene Synonyms | Hsc70t HSP70-1L Hsp70-3 HSP70-HOM HSP70T hum70t Msh5 |
| Gene Full Name | heat shock protein family A (Hsp70) member 1 like |
| Gene Summary | This gene encodes a 70kDa heat shock protein. In conjunction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which also encode isoforms of the 70kDa heat shock protein. [provided by RefSeq, Jul 2008] |
| Alternative Names | epididymis secretory sperm binding protein heat shock 10kDa protein 1-like heat shock 70 kDa protein 1-Hom heat shock 70 kDa protein 1L heat shock 70 kDa protein 3 heat shock 70 kDa-like protein 1 heat shock 70kD protein-like 1 heat shock 70kDa protein 1-like Heat shock protein heat shock protein 1-like heat shock protein 70 Heat shock protein 70-1l heat shock protein cognate 70 testis heat shock protein family A member 1L heat shock-related protein heat shock-related protein hsc70t HSP70.3 inducible heat shock protein 70 spermatid-specific heat shock protein 70 |
| Molecular Weight(MW) | 70(observed：55、65kDa) |
| Cellular Localization | Cytosol,Secreted |
| SwissProt ID | P34931 |
|---|
ELISA
Coat the plate with immunizing antigen.Blue line： Antibody; Orange line: Isotype control antibody
WB
Western blot analysis using HSPA1L antibody against Hela(1),Hepg2(2),K562(3),A549(4) cell,Mouse lung tissue(5),Mouse spleen tissue(6),Mouse stomach tissue(7),Mouse heart tissue(8) . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:2000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.| Application Notes | WB:1:1000-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | PBS ONLY |
