ELISA
Coat the plate with immunizing antigen.Blue line： Antibody; Orange line: Isotype control antibodyWB
Western blot analysis using TSHB antibody against Hela(1), Hepg2(2),K562(3),A549(4),A431(5) cell,Rat brain tissue(6),Rat stomach tissue(7),Rat heart tissue(8) . 15% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:2000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.| Product Name | TSHB Rabbit mAb,pbs only |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| WB:1:1000-1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Antigen peptide affinity chromatography |
| Gene Symbol | TSHB |
|---|---|
| Gene Synonyms | CG-ALPHA FSHA GPA1 GPHa GPHA1 HCG LHA TSH-B TSH-BETA TSHA |
| Gene Full Name | thyroid stimulating hormone subunit beta |
| Gene Summary | The four human glycoprotein hormones chorionic gonadotropin (CG), luteinizing hormone (LH), follicle stimulating hormone (FSH), and thyroid stimulating hormone (TSH) are dimers consisting of alpha and beta subunits that are associated noncovalently. The alpha subunits of these hormones are identical, however, their beta chains are unique and confer biological specificity. Thyroid stimulating hormone functions in the control of thyroid structure and metabolism. The protein encoded by this gene is the beta subunit of thyroid stimulating hormone. Mutations in this gene are associated with congenital central and secondary hypothyroidism and Hashimoto's thyroiditis. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, May 2013] |
| Alternative Names | anterior pituitary glycoprotein hormones common subunit alpha CGA choriogonadotropin alpha chain chorionic gonadotrophin subunit alpha chorionic gonadotropin alpha polypeptide follicle-stimulating hormone alpha chain follicle-stimulating hormone alpha subunit follitropin alpha chain FSH-alpha glycoprotein hormone alpha subunit glycoprotein hormones alpha subunit LSH-alpha luteinizing hormone alpha chain lutropin alpha chain pituitary hormone alpha subunit thyroid stimulating hormone beta thyroid stimulating hormone beta subunit thyroid-stimulating hormone alpha chain Thyrotropin thyrotropin alpha chain thyrotropin beta chain thyrotropin-beta-subunit precursor TSH-alpha TSH-B TSH-beta |
| Molecular Weight(MW) | 16(observed:25kDa) |
| Cellular Localization | Secreted |
| SwissProt ID | P01222 |
|---|
ELISA
Coat the plate with immunizing antigen.Blue line： Antibody; Orange line: Isotype control antibody
WB
Western blot analysis using TSHB antibody against Hela(1), Hepg2(2),K562(3),A549(4),A431(5) cell,Rat brain tissue(6),Rat stomach tissue(7),Rat heart tissue(8) . 15% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:2000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.| Application Notes | WB:1:1000-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | PBS ONLY |
