ELISA
Coat the plate with immunizing antigen.Blue line： Antibody; Orange line: Isotype control antibodyWB
Western blot analysis using BAFF antibody against Mouse stomach tissue(1),Mouse heart tissue(2),Rat brain tissue(3), Rat stomach tissue(4),Rat heart tissue(5) . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:15000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.| Product Name | BAFF Rabbit mAb,pbs only |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| WB:1:1000-1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | TNFSF13B |
|---|---|
| Gene Synonyms | BAFF BLYS CD257 D8Ertd387e TALL-1 TALL1 THANK TNFSF20 TNLG7A ZTNF4 |
| Gene Full Name | TNF superfamily member 13b |
| Gene Summary | The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFFR. This cytokine is expressed in B cell lineage cells, and acts as a potent B cell activator. It has been also shown to play an important role in the proliferation and differentiation of B cells. Alternatively spliced transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Mar 2011] |
| Alternative Names | ApoL related ligand TALL-1 b cell-activating factor B-cell activating factor B-cell-activating factor B-lymphocyte stimulator BAFF TNF family BAFF TNFalpha homolog delta BAFF Delta4 BAFF dendritic cell-derived TNF-like molecule epididymis secretory sperm binding protein TNF and ApoL-related leukocyte expressed ligand 1 TNF family member TNF homolog that activates apoptosis TNF ligand superfamily member TNF-like cytokine TNFSF13B tumor necrosis factor (ligand) superfamily member 13b tumor necrosis factor (ligand) superfamily member 20 tumor necrosis factor ligand 7A tumor necrosis factor superfamily member 13b tumor necrosis factor-like protein ZTNF4 |
| Molecular Weight(MW) | 31(observed：35kDa) |
| Cellular Localization | Secreted, Cell membrane |
| SwissProt ID | Q9Y275 |
|---|
ELISA
Coat the plate with immunizing antigen.Blue line： Antibody; Orange line: Isotype control antibody
WB
Western blot analysis using BAFF antibody against Mouse stomach tissue(1),Mouse heart tissue(2),Rat brain tissue(3), Rat stomach tissue(4),Rat heart tissue(5) . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:15000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.| Application Notes | WB:1:1000-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | PBS ONLY |
