ELISA
Coat the plate with immunizing antigen.Blue line： Antibody; Orange line: Isotype control antibodyWB
Western blot analysis using PRKAG3 antibody against Hela(1),Hepg2(2),K562(3),A549(4),A431(5) cell,Mouse skin tissue(6),Mouse stomach tissue(7) . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:2000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.| Product Name | PRKAG3 Rabbit mAb,pbs only |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| WB:1:1000-1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | PRKAG3 |
|---|---|
| Gene Synonyms | 2410051C13Rik AAKG AAKG2 Amkg3 AMPKG AMPKG3 AMPKg3L AMPKg3S CMH6 GSDH H91620p Prkaac Prkga1 SMGMQTL WPWS |
| Gene Full Name | protein kinase AMP-activated non-catalytic subunit gamma 3 |
| Gene Summary | The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit is one of the gamma regulatory subunits of AMPK. It is dominantly expressed in skeletal muscle. Studies of the pig counterpart suggest that this subunit may play a key role in the regulation of energy metabolism in skeletal muscle. [provided by RefSeq, Jul 2008] |
| Alternative Names | 5'-AMP-activated protein kinase gamma-1 subunit 5'-AMP-activated protein kinase gamma-3 subunit adenosine monophosphate-activated protein kinase gamma 2-subunit AMP-activated protein kinase gamma 3 subunit AMP-activated protein kinase gamma subunit AMP-activated protein kinase gamma2 subunit AMP-activated protein kinase noncatalytic gamma-1 subunit ampk gamma AMPK gamma 2 AMPK gamma-1 chain AMPK gamma-2 chain AMPK gamma-3 chain AMPK gamma1 AMPK gamma3 AMPK subunit gamma-1 AMPK subunit gamma-2 AMPK subunit gamma-3 AMPK- gamma1 AMPK- gamma2 AMPK- gamma3 AMPKg AMPKG3 epididymis secretory sperm binding protein Protein kinase AMP-activated gamma protein kinase AMP-activated gamma 1 non-catalytic subunit protein kinase AMP-activated gamma 2 non-catalytic subunit protein kinase AMP-activated gamma 3 non-catalytic subunit protein kinase AMP-activated gamma 3 non-catatlytic subunit |
| Molecular Weight(MW) | 54(observed:68kDa) |
| Cellular Localization | Cytosol, extracellular space, nucleoplasm, nucleotide-activated protein kinase complex |
| SwissProt ID | Q9UGI9 |
|---|
ELISA
Coat the plate with immunizing antigen.Blue line： Antibody; Orange line: Isotype control antibody
WB
Western blot analysis using PRKAG3 antibody against Hela(1),Hepg2(2),K562(3),A549(4),A431(5) cell,Mouse skin tissue(6),Mouse stomach tissue(7) . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:2000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.| Application Notes | WB:1:1000-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | PBS ONLY |
