ELISA
Coat the plate with immunizing antigen.Blue line： Antibody; Orange line: Isotype control antibodyWB
Western blot analysis using CIR1 antibody against C2C12(1),P19(2),Huvec(3),Mcf-7(4),Raji(5) cell . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:30000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.| Product Name | CIR1 Rabbit mAb,pbs only |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| WB:1:1000-1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | CIRSR |
|---|---|
| Gene Synonyms | CIR CIR1 THE1B/CIR1 |
| Gene Full Name | corepressor of RBPJ and splicing regulator |
| Gene Summary | Enables histone deacetylase binding activity; protein kinase binding activity; and transcription corepressor activity. Acts upstream of or within negative regulation of transcription by RNA polymerase II. Located in centrosome and nuclear speck. Part of protein-containing complex. [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | CBF1 (RBPJ) interacting corepressor 1 CBF1-interacting corepressor corepressor interacting with RBPJ 1 corepressor interacting with RBPJ CIR1 recepin THE1B/CIR1 |
| Molecular Weight(MW) | 52(observed：70kDa) |
| Cellular Localization | Nucleus speckle;Cytoplasm, cytoskeleton, microtubule organizing center, centrosome |
| SwissProt ID | Q86X95 |
|---|
ELISA
Coat the plate with immunizing antigen.Blue line： Antibody; Orange line: Isotype control antibody
WB
Western blot analysis using CIR1 antibody against C2C12(1),P19(2),Huvec(3),Mcf-7(4),Raji(5) cell . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:30000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.| Application Notes | WB:1:1000-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | PBS ONLY |
