WB
Western blot analysis using SLC39A9 antibody against Cos-7(1),B16(2),,THP-1(3),A549(4) cell,Mouse liver tissue(5) . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 30S.ELISA
Coat the plate with immunizing antigen.Blue line： Antibody; Orange line: Isotype control antibody| Product Name | SLC39A9 Rabbit mAb,pbs only |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| WB:1:1000-1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | SLC39A9 |
|---|---|
| Gene Synonyms | 2010002A02Rik 2610511I23Rik 4833420E20Rik ZIP-9 ZIP9 |
| Gene Full Name | solute carrier family 39 member 9 |
| Gene Summary | Enables G protein-coupled receptor activity; androgen binding activity; and zinc efflux transmembrane transporter activity. Involved in intracellular zinc ion homeostasis; regulation of vascular endothelial cell proliferation; and zinc ion transmembrane transport. Located in several cellular components, including mitochondrial membrane; perinuclear region of cytoplasm; and trans-Golgi network. [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | solute carrier family 39 (zinc transporter) member 9 solute carrier family 39 member 9 zinc transporter zinc transporter 9 zinc transporter SLC39A9 ZIP-9 ZIP9 zrt- and Irt-like protein 9 ZRT/IRT-like protein 9 |
| Molecular Weight(MW) | 32kDa（observed：40kDa) |
| Cellular Localization | Golgi apparatus, trans-Golgi network membrane;Cell membrane;Cytoplasm, perinuclear region;Mitochondrion;Nucleus |
| SwissProt ID | Q9NUM3 |
|---|
WB
Western blot analysis using SLC39A9 antibody against Cos-7(1),B16(2),,THP-1(3),A549(4) cell,Mouse liver tissue(5) . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 30S.
ELISA
Coat the plate with immunizing antigen.Blue line： Antibody; Orange line: Isotype control antibody| Application Notes | WB:1:1000-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | PBS ONLY |
