WB
Western blot analysis using SLC4A3 antibody against Hela(1),Nih3t3(2) cell . 8% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.ELISA
Coat the plate with immunizing antigen.Blue line： Antibody; Orange line: Isotype control antibody| Product Name | SLC4A3 Rabbit mAb,pbs only |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| WB:1:1000-1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | SLC4A3 |
|---|---|
| Gene Synonyms | A930038D23Rik AE3 Aep3 CAE3/BAE3 SLC2C SQT7 |
| Gene Full Name | solute carrier family 4 member 3 |
| Gene Summary | The protein encoded by this gene is a plasma membrane anion exchange protein. The encoded protein has been found in brain, heart, kidney, small intestine, and lung. [provided by RefSeq, May 2016] |
| Alternative Names | AE 3 AE anion exchanger 14-AE3 isoform AE3 protein anion exchanger 3 Anion exchanger 3 neuronal B3RP-3 band 3-related protein 3 brain anion exchanger AE3 cardiac/brain band 3-like protein Cl-/HCO3- exchanger (B3RP3) neuronal band 3-like protein solute carrier family 4 (anion exchanger) member 3 solute carrier family 4 member 3 Solute carrier family 4 member 3 anion exchange protein 3 |
| Molecular Weight(MW) | 135kDa（observed：130kDa) |
| Cellular Localization | Cell membrane |
| SwissProt ID | P48751 |
|---|
WB
Western blot analysis using SLC4A3 antibody against Hela(1),Nih3t3(2) cell . 8% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.
ELISA
Coat the plate with immunizing antigen.Blue line： Antibody; Orange line: Isotype control antibody| Application Notes | WB:1:1000-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | PBS ONLY |
