WB
Western blot analysis using ST3GAL3 antibody against Hela(1),Cos-7(2),K562(3),Hepg2(4),A549(5) cell . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.ELISA
Coat the plate with immunizing antigen.Blue line： Antibody; Orange line: Isotype control antibody| Product Name | ST3GAL3 Rabbit mAb,pbs only |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| WB:1:1000-1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | ST3GAL3 |
|---|---|
| Gene Synonyms | DEE15 EIEE15 MRT12 Siat3 SIAT6 ST3Gal III ST3GALII ST3GalIII ST3N |
| Gene Full Name | ST3 beta-galactoside alpha-2,3-sialyltransferase 3 |
| Gene Summary | The protein encoded by this gene is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. The encoded protein is normally found in the Golgi apparatus but can be proteolytically processed to a soluble form. This protein is a member of glycosyltransferase family 29. Mutations in this gene have been associated with a form of autosomal recessive nonsymdromic cognitive disability as well as infantile epileptic encephalopathy. Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq, Jul 2017] |
| Alternative Names | alpha 2 3-sialyltransferase III alpha 2 3-ST 3 alpha-2 3-sialyltransferase II beta-galactoside alpha-2 3-sialyltransferase 3 gal beta-1 3(4) GlcNAc alpha-2 3 sialyltransferase Gal beta-1 3(4)GlcNAc alpha-2 3 sialyltransferase N-acetyllacosaminide alpha 2 3-sialyltransferase N-acetyllactosaminide alpha-2 3-sialyltransferase sialyltransferase (N-acetyllacosaminide alpha 23-sialyltransferase) sialyltransferase 3 sialyltransferase 6 (N-acetyllacosaminide alpha 2 3-sialyltransferase) sialyltransferase 6 (N-acetyllactosaminide alpha 2 3-sialyltransferase) sialyltransferase 6(N-acetyllacosaminide alpha 23-sialyltransferase) |
| Molecular Weight(MW) | 42kDa |
| Cellular Localization | Golgi apparatus, Golgi stack membrane;Secreted |
| SwissProt ID | Q11203 |
|---|
WB
Western blot analysis using ST3GAL3 antibody against Hela(1),Cos-7(2),K562(3),Hepg2(4),A549(5) cell . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.
ELISA
Coat the plate with immunizing antigen.Blue line： Antibody; Orange line: Isotype control antibody| Application Notes | WB:1:1000-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | PBS ONLY |
