WB
Western blot analysis using Cytokeratin 8 mouse monoclonal antibody against Hela(1),Hepg2(2),A549(3),Huvec(4),Cos-7(5),P19(6),B16(7) cell lysate . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Mouse IgG (H&L) - HRP（OM644366）, at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60SIHC
Immunohistochemical analysis of paraffin-embedded breast cancer tissues using Cytokeratin 8 antibody with DAB staining.Pre-treat the sections with heat-mediated antigen retrieval using sodium citrate buffer (pH 6.0) (OM750020) for 2 minutes. Wash the sections with ddH₂O and PBS (OM750003). Block the tissue with 10% non-immune goat serum(OM760028) at room temperature for 30 minutes. Incubate the tissue with the primary antibody diluted at a ratio of 1:1000 at 4°C overnight. At room temperature, dilute the secondary antibody, Goat Anti-Mouse IgG(H&L)-HRP (OM644366), at a ratio of 1:200 and incubate for one hour. Use DAB(OM760029)as the chromogenic agent. Counterstain the tissue with hematoxylin, and mount the tissue sections with neutral gum.IHC
Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using Cytokeratin 8 antibody with DAB staining.Pre-treat the sections with heat-mediated antigen retrieval using sodium citrate buffer (pH 6.0) (OM750020) for 2 minutes. Wash the sections with ddH₂O and PBS (OM750003). Block the tissue with 10% non-immune goat serum(OM760028) at room temperature for 30 minutes. Incubate the tissue with the primary antibody diluted at a ratio of 1:1000 at 4°C overnight. At room temperature, dilute the secondary antibody, Goat Anti-Mouse IgG(H&L)-HRP (OM644366), at a ratio of 1:200 and incubate for one hour. Use DAB(OM760029)as the chromogenic agent. Counterstain the tissue with hematoxylin, and mount the tissue sections with neutral gum.IHC
Immunohistochemical analysis of paraffin-embedded colon cancer tissues using Cytokeratin 8 antibody with DAB staining.Pre-treat the sections with heat-mediated antigen retrieval using sodium citrate buffer (pH 6.0) (OM750020) for 2 minutes. Wash the sections with ddH₂O and PBS (OM750003). Block the tissue with 10% non-immune goat serum(OM760028) at room temperature for 30 minutes. Incubate the tissue with the primary antibody diluted at a ratio of 1:1000 at 4°C overnight. At room temperature, dilute the secondary antibody, Goat Anti-Mouse IgG(H&L)-HRP (OM644366), at a ratio of 1:200 and incubate for one hour. Use DAB(OM760029)as the chromogenic agent. Counterstain the tissue with hematoxylin, and mount the tissue sections with neutral gum.IHC
Immunohistochemical analysis of paraffin-embedded lung cancer tissues using Cytokeratin 8 antibody with DAB staining.Pre-treat the sections with heat-mediated antigen retrieval using sodium citrate buffer (pH 6.0) (OM750020) for 2 minutes. Wash the sections with ddH₂O and PBS (OM750003). Block the tissue with 10% non-immune goat serum(OM760028) at room temperature for 30 minutes. Incubate the tissue with the primary antibody diluted at a ratio of 1:1000 at 4°C overnight. At room temperature, dilute the secondary antibody, Goat Anti-Mouse IgG(H&L)-HRP (OM644366), at a ratio of 1:200 and incubate for one hour. Use DAB(OM760029)as the chromogenic agent. Counterstain the tissue with hematoxylin, and mount the tissue sections with neutral gum.IHC
Immunohistochemical analysis of paraffin-embedded prostate cancer tissues using Cytokeratin 8 antibody with DAB staining.Pre-treat the sections with heat-mediated antigen retrieval using sodium citrate buffer (pH 6.0) (OM750020) for 2 minutes. Wash the sections with ddH₂O and PBS (OM750003). Block the tissue with 10% non-immune goat serum(OM760028) at room temperature for 30 minutes. Incubate the tissue with the primary antibody diluted at a ratio of 1:1000 at 4°C overnight. At room temperature, dilute the secondary antibody, Goat Anti-Mouse IgG(H&L)-HRP (OM644366), at a ratio of 1:200 and incubate for one hour. Use DAB(OM760029)as the chromogenic agent. Counterstain the tissue with hematoxylin, and mount the tissue sections with neutral gum.IF-P
Immunohistochemical analysis of paraffin-embedded human colon cancer tissues using Cytokeratin 8 antibody with DAB staining.Pre-treat the sections with heat-mediated antigen retrieval using sodium citrate buffer (pH 6.0) (OM750020) for 2 minutes. Wash the sections with ddH₂O and PBS (OM750003). Block the tissue with 10% non-immune goat serum(OM760028) at room temperature for 30 minutes. Incubate the tissue with the primary antibody diluted at a ratio of 1:200 at 4°C overnight. At room temperature, dilute the secondary antibody, Goat Anti-Mouse IgG(H&L)-HRP (OM644366), at a ratio of 1:200 and incubate for one hour. Tyramine labeled with 488 (Green,OM642679) was used as chromogenic agent, and DAPI(Blue,OM642679) was used for double dyeing, and the anti-fluorescence attenuation tablets were sealed.ELISA
Blue line: Antibody; Orange line: Isotype control antibody| Product Name | Anti-Cytokeratin 8 antibody,pbs only |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Mouse |
| Tested Applications | ELISAIF-PIHCWB |
| WB:1:1000 IHC:1:1000 IF-P:1:200-1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | KRT8 |
|---|---|
| Gene Synonyms | 2310001L23Rik 2310016L08Rik 3300001P10Rik 39.1 4732484G22Rik AEI2 BCIE BIE CARD2 CK 2e CK-10 CK-14 CK-16 CK-17 CK-18 CK-19 CK-1B CK-2e CK-3 CK-4 CK-5 CK-6A CK-6C CK-6E CK-8 CK1 CK10 CK13 CK14 CK15 CK16 CK18 CK19 CK3 CK4 CK5 CK6A CK6C CK6D CK7 CK8 CYK18 CYK4 CYK8 CYKER D130054E02Rik D15Wsu77e DDD DDD1 EBS1 EBS1A EBS1B EBS1C EBS1D EBS2 EBS2A EBS2B EBS2C EBS2D EBS2E EBS2F EBS3 EBS4 EHK EHK1 EHK2 EHK2A EHK2B EndoA EndoC EPPK FNEPPK GK-19 HUMCYT2A IHL K1 K10 K13 K14 K15 K16 K17 K18 K19 K1B K1C1 K1CO K1CP K1CS K1emb K2C7 K2C8 K2E K3 K4 K5 K6[a] K6A K6C K6D K7 K76 K77 K8 Ka10 Ka13 Ka14 Ka15 Ka16 Ka17 Ka19 Kb1 Kb2 Kb39 Kb4 Kb9 KER1 Ker10 keratin keratin-19 KERIA KERIB KO KPP Krt-1.10 Krt-1.13 Krt-1.14 Krt-1.19 Krt-2.1 Krt-2.4 Krt-2.8 Krt1-10 Krt1-13 Krt1-14 Krt1-15 Krt1-16 Krt1-17 Krt1-18 Krt1-19 KRT13 Krt14 Krt14l KRT15 KRT16A KRT19 KRT1A KRT1B Krt2-1 Krt2-17 Krt2-2 Krt2-4 Krt2-5 Krt2-6a Krt2-6c Krt2-7 KRT2-8 Krt239 KRT2A KRT2B KRT2E KRT2P KRT5A KRT6C KRT6D Krt86 KRTE MECD2 mK1b mK6[a] MK6a NEPPK NFJ PC1 PC2 PC3 PCHC1 Sak57 SCL Tfip8 TROMA-1 WSN1 WSN2 |
| Gene Full Name | keratin 8 |
| Gene Summary | This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012] |
| Alternative Names | {"title":"Alternative Names" "key":"AlternativeNames" "value":"40-kDa keratin intermediate filament 47 kDa cytokeratin 56 kDa cytokeratin 57kd keratin 57kDa keratin 58 kDa cytokeratin 60-kDa keratin 65 kDa cytokeratin 67 kDa cytokeratin 70 kd type II alpha keratin cell proliferation-inducing gene 46 protein CK-1 CK-10 CK-13 CK-14 CK-15 CK-17 CK-18 CK-19 CK-1B CK-2e CK-2P CK-3 CK-4 CK-5 CK-7 CK-8 cytokeratin 1 cytokeratin 10 cytokeratin 13 cytokeratin 14 cytokeratin 15 cytokeratin 16 cytokeratin 18 cytokeratin 19 cytokeratin 2 cytokeratin 3 cytokeratin 4 cytokeratin 5 cytokeratin 6A cytokeratin 6C cytokeratin 6D Cytokeratin 7 cytokeratin 8 cytokeratin endo A cytokeratin endo B cytokeratin otokeratin cytokeratin type II component Ia cytokeratin type II component Ib/c cytokeratin type II component III cytokeratin VIB cytokeratin VII cytokeratin-1 cytokeratin-10 cytokeratin-13 cytokeratin-14 cytokeratin-15 cytokeratin-16 cytokeratin-17 cytokeratin-18 cytokeratin-19 cytokeratin-1B cytokeratin-2e cytokeratin-2P cytokeratin-3 cytokeratin-4 cytokeratin-5 cytokeratin-6A cytokeratin-6B cytokeratin-7 cytokeratin-8 cytokeratin-A cytoplasmic intermediate filament protein cytoskeletal 57 kDa keratin cytoskeletal protein ear specific cytokeratin embryonic type II keratin 1 EndoA cytokeratin (5' end put.) putative EndoA' cytokeratin (5' end put.) putative epidermal keratin 10 epidermal keratin type I epidermal keratin VII epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types epidermolytic hyperkeratosis 1 epidermolytic hyperkeratosis keratosis palmaris et plantaris epithelial keratin 1 epithelial keratin 10 epithelial keratin 2e epithelial keratin-2e focal non-epidermolytic palmoplantar keratoderma hair alpha protein HLA-DRB1-restricted epitope S1 HLA-DRB1-restricted epitope S2 HLA-DRB1-restricted epitope S3 HLA-DRB1-restricted epitope S4 HMWCK intermediate filament intermediate filament protein intermediate filament protein K16 intermediate filament protein K7 K1 K10 K13 K14 K15 K17 K18 K19 K1B K2e K2P K3 keratin K5 K6-alpha K7 K76 K77 K8 KERA keratin 1 (epidermolytic hyperkeratosis) keratin 1 type II keratin 10 (epidermolytic hyperkeratosis) keratin 10 (epidermolytic hyperkeratosis keratosis palmaris et plantaris) keratin 10 type I keratin 13 keratin 13 type I keratin 14 keratin 14 (epidermolysis bullosa simplex Dowling-Meara Koebner) keratin 14 type I keratin 14-like keratin 15 keratin 15 type I keratin 16 (focal non-epidermolytic palmoplantar keratoderma) keratin 16 homolog keratin 16 type I keratin 17 type I keratin 18 type I keratin 19 keratin 19 type I Keratin 1B keratin 2 epidermis keratin 2 type II keratin 2A (epidermal ichthyosis bullosa of Siemens) keratin 2p keratin 3 type II keratin 4 type II keratin 5 (epidermolysis bullosa simplex Dowling-Meara/Kobner/Weber-Cockayne types) keratin 5 type II keratin 6 alpha keratin 6A type II keratin 6A type II keratin 7 type II keratin 76 type II keratin 77 type II keratin 8 keratin 8 type II keratin 86 keratin complex 1 acidic gene 10 keratin complex 1 acidic gene 13 keratin complex 1 acidic gene 14 keratin complex 1 acidic gene 15 keratin complex 1 acidic gene 16 keratin complex 1 acidic gene 17 keratin complex 1 acidic gene 18 keratin complex 1 acidic gene 19 keratin complex 2 basic gene 1 keratin complex 2 basic gene 17 keratin complex 2 basic gene 39 keratin complex 2 basic gene 4 keratin complex 2 basic gene 5 keratin complex 2 basic gene 6a keratin complex 2 basic gene 6c keratin complex 2 basic gene 7 keratin complex 2 basic gene 8 keratin complex 2 gene 17 keratin D keratin intermediate filament 16a keratin intermediate filament 16b keratin IV keratin K3 keratin K5 keratin type II Keratin type II cytoskeletal 5 keratin 55K type II cytoskeletal keratin epidermal type II K6A keratin simple epithelial type I K7 keratin type I cytoskeletal 59 kDa keratin type I 40-kd keratin type II cytoskeletal 60 kDa component III keratin type II cytoskeletal 6A keratin-10 keratin-15 basic keratin-15 beta keratin-2 epidermis keratin-2e keratin-6-alpha KRT1B KRT5 masticatory epithelia keratin 2p mK6-alpha ORF otokeratin Pan CK pan Cytokeratin pan keratin pankeratin PIG46 Sak57 sarcolectin suprabasal cytokeratin 10 tuftelin interacting protein 8 type I alpha-keratin 14 type I alpha-keratin 15 type I cytoskeletal 15 type I keratin KA10 type I keratin KA13 type I keratin Ka14 type I keratin KA15 type I keratin KA16 type I keratin KA17 type I keratin KA19 type II alpha-keratin IIA type II alpha-keratin IIC type II cytokeratin A (no.8) type II cytoskeletal 1b type II cytoskeletal cochleal type II keratin Kb2 type II keratin Kb39 type II keratin Kb9 type II keratin subunit protein type II mesothelial keratin K7 type-II intermediate filament protein type-II keratin Kb1 type-II keratin Kb2 Type-II keratin Kb3 Type-II Keratin Kb39 type-II keratin Kb4 type-II keratin Kb5 type-II keratin Kb6 type-II keratin Kb7 type-II keratin Kb8 type-II keratin Kb9" "issearch":"0"} |
| Molecular Weight(MW) | 52-54kDa |
| Cellular Localization | Cytoplasm, Nucleus cytoplasm |
| SwissProt ID | P05787 |
|---|
WB
Western blot analysis using Cytokeratin 8 mouse monoclonal antibody against Hela(1),Hepg2(2),A549(3),Huvec(4),Cos-7(5),P19(6),B16(7) cell lysate . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Mouse IgG (H&L) - HRP（OM644366）, at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S
IHC
Immunohistochemical analysis of paraffin-embedded breast cancer tissues using Cytokeratin 8 antibody with DAB staining.Pre-treat the sections with heat-mediated antigen retrieval using sodium citrate buffer (pH 6.0) (OM750020) for 2 minutes. Wash the sections with ddH₂O and PBS (OM750003). Block the tissue with 10% non-immune goat serum(OM760028) at room temperature for 30 minutes. Incubate the tissue with the primary antibody diluted at a ratio of 1:1000 at 4°C overnight. At room temperature, dilute the secondary antibody, Goat Anti-Mouse IgG(H&L)-HRP (OM644366), at a ratio of 1:200 and incubate for one hour. Use DAB(OM760029)as the chromogenic agent. Counterstain the tissue with hematoxylin, and mount the tissue sections with neutral gum.
IHC
Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using Cytokeratin 8 antibody with DAB staining.Pre-treat the sections with heat-mediated antigen retrieval using sodium citrate buffer (pH 6.0) (OM750020) for 2 minutes. Wash the sections with ddH₂O and PBS (OM750003). Block the tissue with 10% non-immune goat serum(OM760028) at room temperature for 30 minutes. Incubate the tissue with the primary antibody diluted at a ratio of 1:1000 at 4°C overnight. At room temperature, dilute the secondary antibody, Goat Anti-Mouse IgG(H&L)-HRP (OM644366), at a ratio of 1:200 and incubate for one hour. Use DAB(OM760029)as the chromogenic agent. Counterstain the tissue with hematoxylin, and mount the tissue sections with neutral gum.
IHC
Immunohistochemical analysis of paraffin-embedded colon cancer tissues using Cytokeratin 8 antibody with DAB staining.Pre-treat the sections with heat-mediated antigen retrieval using sodium citrate buffer (pH 6.0) (OM750020) for 2 minutes. Wash the sections with ddH₂O and PBS (OM750003). Block the tissue with 10% non-immune goat serum(OM760028) at room temperature for 30 minutes. Incubate the tissue with the primary antibody diluted at a ratio of 1:1000 at 4°C overnight. At room temperature, dilute the secondary antibody, Goat Anti-Mouse IgG(H&L)-HRP (OM644366), at a ratio of 1:200 and incubate for one hour. Use DAB(OM760029)as the chromogenic agent. Counterstain the tissue with hematoxylin, and mount the tissue sections with neutral gum.
IHC
Immunohistochemical analysis of paraffin-embedded lung cancer tissues using Cytokeratin 8 antibody with DAB staining.Pre-treat the sections with heat-mediated antigen retrieval using sodium citrate buffer (pH 6.0) (OM750020) for 2 minutes. Wash the sections with ddH₂O and PBS (OM750003). Block the tissue with 10% non-immune goat serum(OM760028) at room temperature for 30 minutes. Incubate the tissue with the primary antibody diluted at a ratio of 1:1000 at 4°C overnight. At room temperature, dilute the secondary antibody, Goat Anti-Mouse IgG(H&L)-HRP (OM644366), at a ratio of 1:200 and incubate for one hour. Use DAB(OM760029)as the chromogenic agent. Counterstain the tissue with hematoxylin, and mount the tissue sections with neutral gum.
IHC
Immunohistochemical analysis of paraffin-embedded prostate cancer tissues using Cytokeratin 8 antibody with DAB staining.Pre-treat the sections with heat-mediated antigen retrieval using sodium citrate buffer (pH 6.0) (OM750020) for 2 minutes. Wash the sections with ddH₂O and PBS (OM750003). Block the tissue with 10% non-immune goat serum(OM760028) at room temperature for 30 minutes. Incubate the tissue with the primary antibody diluted at a ratio of 1:1000 at 4°C overnight. At room temperature, dilute the secondary antibody, Goat Anti-Mouse IgG(H&L)-HRP (OM644366), at a ratio of 1:200 and incubate for one hour. Use DAB(OM760029)as the chromogenic agent. Counterstain the tissue with hematoxylin, and mount the tissue sections with neutral gum.
IF-P
Immunohistochemical analysis of paraffin-embedded human colon cancer tissues using Cytokeratin 8 antibody with DAB staining.Pre-treat the sections with heat-mediated antigen retrieval using sodium citrate buffer (pH 6.0) (OM750020) for 2 minutes. Wash the sections with ddH₂O and PBS (OM750003). Block the tissue with 10% non-immune goat serum(OM760028) at room temperature for 30 minutes. Incubate the tissue with the primary antibody diluted at a ratio of 1:200 at 4°C overnight. At room temperature, dilute the secondary antibody, Goat Anti-Mouse IgG(H&L)-HRP (OM644366), at a ratio of 1:200 and incubate for one hour. Tyramine labeled with 488 (Green,OM642679) was used as chromogenic agent, and DAPI(Blue,OM642679) was used for double dyeing, and the anti-fluorescence attenuation tablets were sealed.
ELISA
Blue line: Antibody; Orange line: Isotype control antibody| Application Notes | WB:1:1000 IHC:1:1000 IF-P:1:200-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | PBS ONLY |
