WB
Western blot analysis using CD16 Mouse Monoclonl Antibody against Hepg2(1),A549(2),Cos-7(3),B16(4) cell lysate . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Mouse IgG (H&L) - HRP（OM644366）, at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60SICC/IF
Immunofluorescence analysis of Hela cells using CD16 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Omnimabs® 594-Phalloidin.Cells are fixed in 4% paraformaldehyde at room temperature for 15 minutes. Then, they are permeabilized with a PBS (OM750003) solution containing 0.1% Triton X-100(OM750021) at room temperature for 15 minutes. Subsequently, the cells are blocked with 10% non - immune goat serum(OM760028) at room temperature for 1 hour.The cells are incubated overnight at 4°C with the primary antibody diluted 1:100 in PBS. The secondary antibody, Omnimabs® 488 Goat Ant-Mouse IgG(H&L) (Green,OM643485), is diluted at a ratio of 1:400 and incubated with the cells for 1 hour.Nuclear DNA is labeled with DAPI (Blue,OM643160). F-actin is stained with Omnimabs® 594-Phalloidin (Red,OM750007) diluted 1:100 for 30 minutes.ELISA
Blue line: Antibody; Orange line: Isotype control antibody| Product Name | Anti-CD16 antibody,pbs only |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Mouse |
| Tested Applications | ELISAICC/IFWB |
| WB:1:1000 ICC/IF:1:100-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FCGR3A |
|---|---|
| Gene Synonyms | 4833442P21Rik CD16 CD16-2 CD16-I CD16-II CD16A CD16b FCG3 FcgammaRIV FCGR3 Fcgr3a Fcgr4 FcGRIIIA FcgRIV FCRIIIA FCRIIIb Fcrl3 HNA-1 IGFR3 IMD20 |
| Gene Full Name | Fc gamma receptor IIIa |
| Alternative Names | CD16a antigen CD32 CD32 receptor 2 CDw32 Fc fragment of IgG intermediate affinity IV receptor Fc fragment of IgG low affinity IIIa receptor (CD16a) isoform FCGR3A_1 Fc fragment of IgG low affinity IIIa receptor (CD16a) isoform FCGR3A_2 Fc fragment of IgG receptor IIIa Fc fragment of IgG receptor IIIb Fc fragment of IgG low affinity IIIa receptor (CD16a) Fc fragment of IgG low affinity IIIa receptor for (CD16) Fc fragment of IgG low affinity IIIa receptor for (CD16) Fc fragment of IgG low affinity III receptor for (CD16) Fc fragment of IgG low affinity IIIb receptor (CD16b) Fc gamma receptor III Fc gamma receptor III-A Fc gamma receptor III-B Fc gamma receptor IIIa Fc gammaRIV Fc receptor-like 3 Fc-gamma receptor IIIb (CD16) Fc-gamma RII-b Fc-gamma RII-c fc-gamma RIII Fc-gamma RIII-alpha fc-gamma RIII-beta fc-gamma RIIIb Fc-gamma-R-alpha receptor Fc-gamma-RIIb Fc-gamma-RIIc Fcg receptor III FCG2 FcgammaRIII FcgammaRIIIA FcgammaRIIIb fcgammaRIV FCGR2 FCGR2A FCGR2B FCGR2C FcgRIIIa FcRII-b FcRII-c fcRIII human neutrophil antigen 1 igG Fc receptor III igG Fc receptor III-1 igG Fc receptor III-2 IgG Fc receptor III-A IgG receptor precursor immunoglobulin G Fc receptor III integral to membrane low affinity IgG receptor low affinity IgG receptor CD16 low affinity IgG receptor precursor low affinity immunoglobulin gamma Fc region receptor IV low affinity immunoglobulin gamma receptor III-a Fc fragment neutrophil-specific antigen NA RP11-5K23.1 transmembrane receptor FcgammaRIII-X |
| Molecular Weight(MW) | 50-70kDa |
| Cellular Localization | Cell membrane |
| SwissProt ID | P08637 |
|---|
WB
Western blot analysis using CD16 Mouse Monoclonl Antibody against Hepg2(1),A549(2),Cos-7(3),B16(4) cell lysate . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Mouse IgG (H&L) - HRP（OM644366）, at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S
ICC/IF
Immunofluorescence analysis of Hela cells using CD16 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Omnimabs® 594-Phalloidin.Cells are fixed in 4% paraformaldehyde at room temperature for 15 minutes. Then, they are permeabilized with a PBS (OM750003) solution containing 0.1% Triton X-100(OM750021) at room temperature for 15 minutes. Subsequently, the cells are blocked with 10% non - immune goat serum(OM760028) at room temperature for 1 hour.The cells are incubated overnight at 4°C with the primary antibody diluted 1:100 in PBS. The secondary antibody, Omnimabs® 488 Goat Ant-Mouse IgG(H&L) (Green,OM643485), is diluted at a ratio of 1:400 and incubated with the cells for 1 hour.Nuclear DNA is labeled with DAPI (Blue,OM643160). F-actin is stained with Omnimabs® 594-Phalloidin (Red,OM750007) diluted 1:100 for 30 minutes.
ELISA
Blue line: Antibody; Orange line: Isotype control antibody| Application Notes | WB:1:1000 ICC/IF:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | PBS ONLY |
