WB
Western blot analysis using ATG5 mouse monoclonal antibody against Hela(1),Hepg2(2),A549(3),Huvec(4),Cos-7(5),P19(6),B16(7) cell lysate . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Mouse IgG (H&L) - HRP（OM644366）, at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60SELISA
Blue line: Antibody; Orange line: Isotype control antibody| Product Name | Anti-ATG5 antibody,pbs only |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| WB:1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ATG5 |
|---|---|
| Gene Synonyms | 2010107M05Rik 3110067M24Rik APG5 APG5-LIKE APG5L ASP Atg-5 ATG5 Atg5l CG1643 DmAtg5 Dmel\CG1643 Dmel_CG1643 hAPG5 Paddy SCAR25 XELAEV_18027227mg zgc:100934 |
| Gene Full Name | autophagy related 5 |
| Gene Summary | The protein encoded by this gene, in combination with autophagy protein 12, functions as an E1-like activating enzyme in a ubiquitin-like conjugating system. The encoded protein is involved in several cellular processes, including autophagic vesicle formation, mitochondrial quality control after oxidative damage, negative regulation of the innate antiviral immune response, lymphocyte development and proliferation, MHC II antigen presentation, adipocyte differentiation, and apoptosis. Several transcript variants encoding different protein isoforms have been found for this gene. [provided by RefSeq, Sep 2015] |
| Alternative Names | alternatively spliced APG 5 APG5 APG5 autophagy 5 like (APG5L) APG5 autophagy 5-like apoptosis-specific protein Apoptosis-specific protein (ASP) AT5G17290 ATATG5 ATG5 ATG5 autophagy related 5 homolog Atg5-PA AUTOPHAGY 5 autophagy 5-like protein autophagy protein 5 autophagy related 5-like protein autophagy-specific gene 5 CG1643-PA LD34980p MKP11.20 MKP11_20 similar to S. cerevisiae ATG5 autophagy related 5 |
| Molecular Weight(MW) | 32 kDa(Observed band size: 55 kDa) |
| Cellular Localization | Cytoplasm, Membrane. |
| SwissProt ID | Q9H1Y0 |
|---|
WB
Western blot analysis using ATG5 mouse monoclonal antibody against Hela(1),Hepg2(2),A549(3),Huvec(4),Cos-7(5),P19(6),B16(7) cell lysate . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Mouse IgG (H&L) - HRP（OM644366）, at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S
ELISA
Blue line: Antibody; Orange line: Isotype control antibody| Application Notes | WB:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | PBS ONLY |
