WB
Western blot analysis using PI3 Kinase P85 mouse monoclonal antibody against Hela(1),Hepg2(2),A549(3),Huvec(4),Cos-7(5),P19(6),B16(7) cell lysate . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Mouse IgG (H&L) - HRP（OM644366）, at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60SELISA
Blue line: Antibody; Orange line: Isotype control antibody| Product Name | Anti-PI3 Kinase P85 antibody,pbs only |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| WB:1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PIK3R1 |
|---|---|
| Gene Synonyms | AGM7 GRB1 IMD36 p50alpha p55alpha p85 p85-ALPHA p85alpha PI3K PI3KA |
| Gene Full Name | phosphoinositide-3-kinase regulatory subunit 1 |
| Gene Summary | Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011] |
| Alternative Names | growth factor receptor bound 1 p55-GAMMA p55PIK p85-alpha GRB1 phosphatidylinositol 3-kinase 55 kDa regulatory subunit gamma phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha phosphatidylinositol 3-kinase p85 alpha phosphatidylinositol 3-kinase regulatory subunit gamma phosphatidylinositol 3-kinase regulatory subunit polypeptide 1 (p85 alpha) phosphatidylinositol 3-kinase-associated p-85 alpha Phosphoinositide 3-kinase p85 (other splicing variants: p55 and p50) Phosphoinositide 3-kinase regulatory subunit polypeptide 1 (p85 alpha) phosphoinositide-3-kinase regulatory subunit alpha phosphoinositide-3-kinase regulatory subunit 1 (alpha) phosphoinositide-3-kinase regulatory subunit 1 (p85 alpha) phosphoinositide-3-kinase regulatory subunit polypeptide 1 (p85 alpha) PI3 kinase-associated p85 PI3-kinase p85 subunit alpha PI3-kinase p85-subunit alpha PI3-kinase regulatory subunit alpha PI3-kinase regulatory subunit gamma PI3-kinase subunit p55-gamma PI3-kinase subunit p85-alpha PI3K regulatory subunit alpha PI3K regulatory subunit gamma PI3K-p46 polypeptide 1 p85 alpha ptdIns-3-kinase p85-alpha ptdIns-3-kinase regulatory subunit alpha PtdIns-3-kinase regulatory subunit p85-al ptdIns-3-kinase regulatory subunit p85-alpha RP4-533D7.2 |
| Molecular Weight(MW) | 85kDa |
| Cellular Localization | Cytosol, Membrane, Nucleus. |
| SwissProt ID | P27986 |
|---|
WB
Western blot analysis using PI3 Kinase P85 mouse monoclonal antibody against Hela(1),Hepg2(2),A549(3),Huvec(4),Cos-7(5),P19(6),B16(7) cell lysate . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Mouse IgG (H&L) - HRP（OM644366）, at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S
ELISA
Blue line: Antibody; Orange line: Isotype control antibody| Application Notes | WB:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | PBS ONLY |
