WB
Western blot analysis using P38 antibody against Hela(1),Hepg2(2),A549(3),Huvec(4),Cos-7(5),P19(6),B16(7) lysate . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Mouse IgG (H&L) - HRP（OM644366）, at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60SELISA
Blue line: Antibody; Orange line: Isotype control antibody| Product Name | Anti-P38 antibody,pbs only |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| WB:1:1000-1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MAPK14 |
|---|---|
| Gene Synonyms | c-Crk Crk-I Crk-II Crk-III CRK1 Crk3 CRKII CrkIII Crko CSBP CSBP1 CSBP2 CSPB1 EXIP Hog Mxi2 p38 p38-alpha p38a p38ALPHA p38Hog p38MAPK PRKM14 PRKM15 RK SAPK2A |
| Gene Full Name | mitogen-activated protein kinase 14 |
| Gene Summary | The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008] |
| Alternative Names | avian sarcoma virus CT10 (v-crk) oncogene homolog CRK-II Crk3 CSAID-binding protein Csaids binding protein CT-10 related kinase 3 cytokine suppressive anti-inflammatory drug binding protein cytokine-supressive anti-inflammatory drug binding protein encoded by transcript variant 4 p38 MAP kinase encoded by transcript variant 5 p38 MAP kinase MAP kinase 14 MAP kinase 2 MAP kinase Mxi2 MAP kinase p38 alpha MAPK 14 MAPK14 MAX-interacting protein 2 mitogen-activated protein kinase 14A mitogen-activated protein kinase p38 alpha negative regulator of Crk tyrosine-phosphorylation p38 p38 MAP kinase p38 MAP kinase alpha p38 MAPK p38 mitogen activated protein kinase p38alpha Exip proto-oncogene C-crk reactive kinase SAPK2a CSAIDS binding protein 1 stress-activated protein kinase 2A v-crk avian sarcoma virus CT10 oncogene homolog v-crk sarcoma virus CT10 oncogene homolog v-crk sarcoma virus CT10 oncogene homolog (avian) isoform CRK_1 v-crk sarcoma virus CT10 oncogene homolog (avian) isoform CRK_2 v-crk sarcoma virus CT10 oncogene-like protein |
| Molecular Weight(MW) | 41kDa |
| Cellular Localization | Cytoplasm,Nucleus. |
| SwissProt ID | Q16539 |
|---|
WB
Western blot analysis using P38 antibody against Hela(1),Hepg2(2),A549(3),Huvec(4),Cos-7(5),P19(6),B16(7) lysate . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Mouse IgG (H&L) - HRP（OM644366）, at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S
ELISA
Blue line: Antibody; Orange line: Isotype control antibody| Application Notes | WB:1:1000-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | PBS ONLY |
