WB
Western blot analysis using NF kB P50 antibody against Mcf-7(1) lysate . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Mouse IgG (H&L) - HRP（OM644366）, at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60SELISA
Blue line: Antibody; Orange line: Isotype control antibody| Product Name | Anti-NF kB P50 antibody,pbs only |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| WB:1:1000-1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CVID12 EBP-1 KBF1 NF-kappa-B1 NF-kappaB NF-kappaB1 NF-kappabeta NF-kB NF-KB1 NFkappaB NFKB-p105 NFKB-p50 p105 p50 p50/p105 |
|---|---|
| Alternative Names | DKFZp686C01211 DNA-binding factor KBF1 EBP-1 MGC54151 NF kappaB1 NF KB NF-kappa-B1 p84/NF-kappa-B1 p98 NF-kappaB p50 NF-kB p50/p105 nuclear factor nuclear factor kappa b nuclear factor kappa-B DNA binding subunit nuclear factor kappaB p50 nuclear factor NF-kappa-B p50 nuclear factor NF-kappa-B p50 subunit nuclear factor of kappa light chain gene enhancer in B-cells 1 p105 nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 p105 p105 p50 subunit of NF kappaB p50/p105 transcription factor p105 NF-kappa B precursor |
| Molecular Weight(MW) | 50kDa |
| SwissProt ID | P19838 |
|---|
WB
Western blot analysis using NF kB P50 antibody against Mcf-7(1) lysate . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Mouse IgG (H&L) - HRP（OM644366）, at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S
ELISA
Blue line: Antibody; Orange line: Isotype control antibody| Application Notes | WB:1:1000-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | PBS ONLY |
