WB
Western blot analysis using TRP2 DCT (2A10) antibody against Mouse lung tissue(1) lysate . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Mouse IgG (H&L) - HRP（OM644366）, at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60SELISA
Blue line: Antibody; Orange line: Isotype control antibody| Product Name | Anti-TRP2 DCT antibody,pbs only |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| WB:1:1000-1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DCT |
|---|---|
| Gene Synonyms | DT OCA8 RGD1564975 slaty slt TRP-2 TRP2 Tyrp-2 TYRP2 |
| Gene Full Name | dopachrome tautomerase |
| Gene Summary | Predicted to enable dopachrome isomerase activity. Involved in response to blue light. Located in intracellular membrane-bounded organelle and plasma membrane. Implicated in oculocutaneous albinism. [provided by Alliance of Genome Resources, Apr 2025] |
| Alternative Names | DOPAchrome conversion factor dopachrome delta-isomerase DOPAchrome isomerase DOPAchrome oxidoreductase dopachrome tautomerase dopachrome tautomerase (dopachrome delta-isomerase tyrosine-related protein 2) DT L-dopachrome Delta-isomerase L-dopachrome isomerase SLATY locus protein TRP2 tyrosinase related protein-2 tyrosinase-related protein 2 tyrosinase-related protein-2 tyrosine-related protein 2 |
| Molecular Weight(MW) | 60kDa |
| Cellular Localization | Melanosome membrane,Melanosome. |
| SwissProt ID | P40126 |
|---|
WB
Western blot analysis using TRP2 DCT (2A10) antibody against Mouse lung tissue(1) lysate . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Mouse IgG (H&L) - HRP（OM644366）, at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S
ELISA
Blue line: Antibody; Orange line: Isotype control antibody| Application Notes | WB:1:1000-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | PBS ONLY |
