WB
Western blot analysis using NFAT5 antibody against Hela(1),A549(2),Hepg2(3),Mcf-7(4),NIh3t3(5),Jurkat(6),Hek293(7),P19(8),B16(9),Raji(10),T24(11) lysate . 6% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Mouse IgG (H&L) - HRP（OM644366）, at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60SELISA
Blue line: Antibody; Orange line: Isotype control antibody| Product Name | Anti-NFAT5 antibody,pbs only |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| WB:1:1000-1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NFAT5 |
|---|---|
| Gene Synonyms | B130038B15Rik CAG-8 CAG80 mKIAA0827 NF-AT5 NFATL1 NFATZ OREBP TONEBP |
| Gene Full Name | nuclear factor of activated T cells 5 |
| Gene Summary | The product of this gene is a member of the nuclear factors of activated T cells family of transcription factors. Proteins belonging to this family play a central role in inducible gene transcription during the immune response. This protein regulates gene expression induced by osmotic stress in mammalian cells. Unlike monomeric members of this protein family, this protein exists as a homodimer and forms stable dimers with DNA elements. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Alternative Names | glutamine rich protein H65 NFAT-like protein 1 NFAT5b nuclear factor of activated T-cells 5 tonicity-responsive osmotic response element-binding protein rel domain-containing transcription factor NFAT5 rel similarity domain containing protein T-cell transcription factor NFAT5 TonE-binding protein TonEBP transcription factor tonicity-responsive enhancer binding protein tonicity-responsive enhancer-binding protein transcription factor NFAT5 |
| Molecular Weight(MW) | 166kDa |
| Cellular Localization | Chromosome,Cytoplasm,Nucleus. |
| SwissProt ID | O94916 |
|---|
WB
Western blot analysis using NFAT5 antibody against Hela(1),A549(2),Hepg2(3),Mcf-7(4),NIh3t3(5),Jurkat(6),Hek293(7),P19(8),B16(9),Raji(10),T24(11) lysate . 6% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Mouse IgG (H&L) - HRP（OM644366）, at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S
ELISA
Blue line: Antibody; Orange line: Isotype control antibody| Application Notes | WB:1:1000-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | PBS ONLY |
