WB
Western blot analysis using TrkC antibody against Hela(1),Hepg2(2),K562(3),A549(4),A431(5),Huvec(6),Nih3t3(7),Cos-7(8),C2C12(9) cell . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:2000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.| Product Name | Anti-TrkC antibody,pbs only |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| WB:1:1000-1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | NTRK3 |
|---|---|
| Gene Synonyms | DEE58 EIEE58 gp145(trkC) GP145-TrkB GP145-TrkB/GP95-TrkB GP145-TrkC MTC Ntrk3_tv3 OBHD p140-TrkA RATTRKB1 Tkr Tkrb TRK Trk-A trk-B TRK1 TRKA TRKB TRKB1 TRKC |
| Gene Full Name | neurotrophic receptor tyrosine kinase 3 |
| Gene Summary | This gene encodes a member of the neurotrophic tyrosine receptor kinase (NTRK) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Signalling through this kinase leads to cell differentiation and may play a role in the development of proprioceptive neurons that sense body position. Mutations in this gene have been associated with medulloblastomas, secretory breast carcinomas and other cancers. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2011] |
| Alternative Names | BDNF-tropomyosine receptor kinase B ETS related protein-neurotrophic receptor tyrosine kinase fusion protein ETV6-NTRK3 fusion gp140trk GP145-TrkB/GP95-TrkB GP145-TrkC mTrkC NC1 mTrkC NC2 neural receptor protein-tyrosine kinase (trkB) neural receptor protein-tyrosine kinase (trkC) neurotrophic tyrosine kinase receptor type 2 neurotrophic tyrosine kinase receptor type 1 neurotrophic tyrosine kinase receptor type 2 neurotrophic tyrosine kinase receptor type 3 neurotrophic tyrosine receptor kinase type 2 neurotrophin-3 NTRK2 NTRK3 Oncogene TRK p140-TrkA slow nerve growth factor receptor slow nerve growth factor receptor TRKC(ki39) splice variant splicing variant of the Ntrk3 receptor TEL-TrkC fusion protein trk tyrosine-specific protein kinase trk-A trk-C TRK1-transforming tyrosine kinase protein TrkA neurotrophin receptor trkA proto-oncogene receptor TrkB trkB tyrosine kinase TrkB-T-Shc trkB.T1 TrkC trkC tyrosine kinase TrkC(ki14) slow nerve growth factor receptor tropomyosin receptor kinase A tropomyosin-related kinase A tropomyosin-related kinase B truncated trkB tyrosine kinase receptor A tyrosine kinase receptor B tyrosine kinase receptor C tyrosine protein kinase B (trkB) precursor |
| Molecular Weight(MW) | 94kDa(observed：100kDa) |
| Cellular Localization | Membrane |
| SwissProt ID | Q16288 |
|---|
WB
Western blot analysis using TrkC antibody against Hela(1),Hepg2(2),K562(3),A549(4),A431(5),Huvec(6),Nih3t3(7),Cos-7(8),C2C12(9) cell . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:2000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.
ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.| Application Notes | WB:1:1000-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | PBS ONLY |
