WB
Western blot analysis using Ndufs4 antibody against Rat heart tissue(1) . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:2000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.| Product Name | Anti-Ndufs4 antibody,pbs only |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| WB:1:1000-1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | NDUFS4 |
|---|---|
| Gene Synonyms | 6720411N02Rik AQDQ C1-18k CI-18 CI-18 kDa CI-AQDQ MC1DN1 |
| Gene Full Name | NADH:ubiquinone oxidoreductase subunit S4 |
| Gene Summary | This gene encodes an nuclear-encoded accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (complex I, or NADH:ubiquinone oxidoreductase). Complex I removes electrons from NADH and passes them to the electron acceptor ubiquinone. Mutations in this gene can cause mitochondrial complex I deficiencies such as Leigh syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015] |
| Alternative Names | CI-18 kDa CI-18(IP) CI-AQDQ complex I 18kDa subunit complex I-18 kDa complex I-AQDQ mitochondrial respiratory chain complex I (18-KD subunit) NADH dehydrogenase (ubiquinone) Fe-S protein 4 18kDa (NADH-coenzyme Q reductase) isoform NDUFS4_1 NADH dehydrogenase (ubiquinone) Fe-S protein 4 18kDa (NADH-coenzyme Q reductase) isoform NDUFS4_2 NADH dehydrogenase (ubiquinone) Fe-S protein 4 18kDa (NADH-coenzyme Q reductase) NADH-ubiquinone oxidoreductase 18 kDa subunit subunit of Mitochondrial Complex I |
| Molecular Weight(MW) | 20kDa |
| Cellular Localization | Mitochondrion |
| SwissProt ID | O43181 |
|---|
WB
Western blot analysis using Ndufs4 antibody against Rat heart tissue(1) . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:2000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.
ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.| Application Notes | WB:1:1000-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | PBS ONLY |
