WB
Western blot analysis using METTL1 antibody against Hela(1),K562(2),A549(3),A431(4),Huvec(5),Nih3t3(6),Cos-7(7),C2C12(8),Hek293(9),Mcf-7(10),Pc-12(11),Jurkat(12),Raji(13) cell . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:2000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.| Product Name | Anti-METTL1 antibody,pbs only |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| WB:1:1000-1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | METTL1 |
|---|---|
| Gene Synonyms | 2810012D02Rik C12orf1 TRM8 TRMT8 YDL201w |
| Gene Full Name | methyltransferase 1, tRNA methylguanosine |
| Gene Summary | This gene is similar in sequence to the S. cerevisiae YDL201w gene. The gene product contains a conserved S-adenosylmethionine-binding motif and is inactivated by phosphorylation. Alternative splice variants encoding different protein isoforms have been described for this gene. A pseudogene has been identified on chromosome X. [provided by RefSeq, Jul 2008] |
| Alternative Names | D1075-like D1075-like gene product methyltransferase like 1 methyltransferase-like protein 1 miRNA (guanine-N(7)-)-methyltransferase mRNA (guanine-N(7)-)-methyltransferase tRNA (guanine(46)-N(7))-methyltransferase tRNA(m7G46)-methyltransferase |
| Molecular Weight(MW) | 31kDa |
| Cellular Localization | Nucleus |
| SwissProt ID | Q9UBP6 |
|---|
WB
Western blot analysis using METTL1 antibody against Hela(1),K562(2),A549(3),A431(4),Huvec(5),Nih3t3(6),Cos-7(7),C2C12(8),Hek293(9),Mcf-7(10),Pc-12(11),Jurkat(12),Raji(13) cell . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:2000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.
ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.| Application Notes | WB:1:1000-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | PBS ONLY |
