WB
Western blot analysis using Nudel antibody against K562(1),A549(2),A431(3),Huvec(4),Cos-7(5),Jurkat(6) cell . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:2000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.| Product Name | Anti-Nudel antibody,pbs only |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| WB:1:1000-1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | NDEL1 |
|---|---|
| Gene Synonyms | 2600006O07Rik EOPA MITAP1 mNudE-L mNudel NDE1L1 NDE2 NUDE2 NUDEL |
| Gene Full Name | nudE neurodevelopment protein 1 like 1 |
| Gene Summary | Enables identical protein binding activity. Involved in chromosome segregation; positive regulation of GTPase activity; and regulation of intracellular protein transport. Located in kinetochore. Biomarker of schizophrenia. [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | DKFZp451M0318 endooligopeptidase A LIS1-interacting protein NUDEL LIS1-interacting protein NUDEL endooligopeptidase A mitosin-associated protein 1 mitosin-associated protein MITAP1 nuclear disribution gene E like nuclear distribution gene E-like homolog 1 nudE nuclear distribution E homolog-like 1 nudE nuclear distribution gene E homolog like 1 nudE nuclear distribution gene E homolog-like 1 NudE-L NUDE-like protein protein Nudel |
| Molecular Weight(MW) | 38kDa |
| Cellular Localization | Cytoskeleton |
| SwissProt ID | Q9GZM8 |
|---|
WB
Western blot analysis using Nudel antibody against K562(1),A549(2),A431(3),Huvec(4),Cos-7(5),Jurkat(6) cell . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:2000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.
ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.| Application Notes | WB:1:1000-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | PBS ONLY |
