WB
Western blot analysis using NaV1.7 antibody against Mouse skin tissue(1) . 6% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:2000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.| Product Name | Anti-NaV1.7 antibody,pbs only |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| WB:1:1000-1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | SCN9A |
|---|---|
| Gene Synonyms | 6430408L10 A230052E19Rik B230332M13 BFIC3 BFIS3 BFIS5 BFNIS C630029C19Rik CDCD2 CERIII CIAT CMD1E CMPD2 CMS16 CMYO22A CMYP22A DEE11 DEE13 DEE6 DEE62 DEE6A DEE6B dmu DRVT EA9 EIEE11 EIEE13 EIEE6 EIEE62 ETHA FEB3 FEB3A FEB3B FEPS2 FEPS3 FFEVF4 FHM3 GEFSP2 GEFSP7 Gm1000 HB1 HB2 HBA HBBD HBSCI HBSCII HH1 HOKPP2 HSAN2D HSAN7 HYKPP HYPP ICCD IVF LQT3 MED mH1 mH2 microI mKIAA4197 mnd-2 mnd2 MYOCL2 Na(v)1.2 Na(V)1.4 Na(V)1.8 NAC1 NAC1A NAC2 NAC3 NaCh6 NachII NaN NaT Nav1.1 Nav1.2 Nav1.3 Nav1.4 Nav1.5 Nav1.5c Nav1.6 Nav1.7 Nav1.8 NAV1.9 NCHVS NE-NA NENA nmf2 nmf335 nmf58 NSS2 nur14 PFHB1 PN1 PN3 PN4 PN5 RATRSKM2X RII/RIIA RNSCPIIR rSkM2 RSKM2X SCAL SCIII SCN SCN1 SCN12A Scn2a SCN2A1 SCN2A2 Scn2x ScpII seal SFNP SkM1 SkM2 SMEI SNS SNS-2 SNS2 SSS1 VF1 |
| Gene Full Name | sodium voltage-gated channel alpha subunit 9 |
| Gene Summary | This gene encodes a voltage-gated sodium channel which plays a significant role in nociception signaling. Mutations in this gene have been associated with primary erythermalgia, channelopathy-associated insensitivity to pain, and paroxysmal extreme pain disorder. [provided by RefSeq, Aug 2009] |
| Alternative Names | {"title":"Alternative Names" "key":"AlternativeNames" "value":"3-Feb ataxia 3 brain III voltage-gated sodium channel cardiac tetrodotoxin-insensitive voltage-dependent sodium channel alpha subunit CTC-264K15.6 H1B hH1c voltage-gated sodium channel hNa6/Scn8a voltage-gated sodium channel hNE-Na mu-1 Na+ channel naCh6 NaCh6 Scn8a NaN alpha subunit Nav1.4 Nav1.5/SCN5A.3 NaV1.8 neuroendocrine sodium channel peripheral nerve protein type 4 peripheral nerve sodium channel 3 peripheral nerve sodium channel 5 peripheral sodium channel 1 PN4 rCG26412-like RIIA sodium channel protein SCN10A/PN3 scn5a-JEJ HH1 hNav1.5 LQT3 SCN8A sensory neuron sodium channel sensory neuron sodium channel 2 skeletal muscle sodium channel alpha subunit skeletal muscle voltage-dependent sodium channel type IV alpha subunit skeletal muscle voltage-sensitive sodium channel subtype 2 skM1 sodium channel 25 sodium channel 6 sodium channel alpha-subunit sodium channel protein brain I subunit alpha sodium channel protein brain II subunit alpha sodium channel protein brain III subunit alpha sodium channel protein cardiac muscle subunit alpha sodium channel protein skeletal muscle subunit alpha sodium channel protein type 1 subunit alpha-like sodium channel protein type 3 subunit alpha-like sodium channel protein type I subunit alpha sodium channel protein type II subunit alpha sodium channel protein type III subunit alpha sodium channel protein type IV subunit alpha sodium channel protein type IX subunit alpha sodium channel protein type V subunit alpha sodium channel protein type VIII subunit alpha sodium channel protein type X subunit alpha sodium channel protein type XI subunit alpha sodium channel protein brain I alpha subunit sodium channel protein brain I subunit alpha sodium channel protein brain II subunit alpha sodium channel protein brain III subunit alpha sodium channel protein brain type 2 alpha subunit sodium channel type X alpha polypeptide sodium channel voltage gated type 1 alpha subunit sodium channel voltage-gated type II alpha polypeptide Sodium channel voltage-gated type IV alpha polypeptide sodium channel voltage-gated type IX alpha sodium channel voltage-gated type VIII alpha sodium channel voltage-gated type X alpha polypeptide sodium channel voltage gated type III alpha subunit sodium channel voltage gated type VIII alpha subunit sodium channel voltage-gated type 1 alpha polypeptide sodium channel voltage-gated type 10 alpha polypeptide sodium channel voltage-gated type 11 alpha polypeptide sodium channel voltage-gated type 2 alpha 1 polypeptide sodium channel voltage-gated type 2 alpha 1 subunit sodium channel voltage-gated type 3 alpha subunit sodium channel voltage-gated type 4 alpha polypeptide sodium channel voltage-gated type 4 alpha subunit sodium channel voltage-gated type 5 alpha subunit sodium channel voltage-gated type 8 alpha polypeptide sodium channel voltage-gated type 8 alpha subunit sodium channel voltage-gated type 9 alpha polypeptide sodium channel voltage-gated type I alpha polypeptide sodium channel voltage-gated type I alpha subunit sodium channel voltage-gated type II alpha 1 sodium channel voltage-gated type II alpha 1 polypeptide sodium channel voltage-gated type II alpha 2 polypeptide sodium channel voltage-gated type II alpha polypeptide 1 sodium channel voltage-gated type II alpha subunit sodium channel voltage-gated type III alpha polypeptide sodium channel voltage-gated type III alpha subunit sodium channel voltage-gated type IV alpha polypeptide sodium channel voltage-gated type IV alpha subunit sodium channel voltage-gated type IX alpha polypeptide sodium channel voltage-gated type IX alpha subunit sodium channel voltage-gated type V alpha polypeptide sodium channel voltage-gated type V alpha subunit sodium channel voltage-gated type VIII alpha polypeptide sodium channel voltage-gated type X alpha polypeptide sodium channel voltage-gated type X alpha subunit sodium channel voltage-gated type XI alpha sodium channel voltage-gated type XI alpha polypeptide sodium channel voltage-gated type XI alpha subunit sodium channel voltage-gated type XII alpha polypeptide sodium channel voltage-gated type11 alpha polypeptide sodium ion channel SP-19 splice variant TTX-R sodium channel voltage gated sodium channel voltage gated sodium channel alpha subunit voltage-dependent sodium channel voltage-gated sodium channel alpha subunit voltage-gated sodium channel alpha subunit Nav1.7 voltage-gated sodium channel Nav1.5c voltage-gated sodium channel NAV1.9b voltage-gated sodium channel subtype II voltage-gated sodium channel subtype III voltage-gated sodium channel subunit alpha Nav1.1 voltage-gated sodium channel subunit alpha Nav1.2 voltage-gated sodium channel subunit alpha Nav1.3 voltage-gated sodium channel subunit alpha Nav1.4 voltage-gated sodium channel subunit alpha Nav1.5 voltage-gated sodium channel subunit alpha Nav1.6 voltage-gated sodium channel subunit alpha Nav1.7 voltage-gated sodium channel subunit alpha Nav1.8 voltage-gated sodium channel subunit alpha Nav1.9 voltage-gated sodium channel type II alpha subunit voltage-gated sodium channel type VIII alpha protein voltage-gated sodium channel alpha subunit domain 4 S5-S6 voltage-sensitive sodium channel alpha subunit" "issearch":"0"} |
| Molecular Weight(MW) | 226kDa |
| Cellular Localization | Cell membrane, Cell projection |
| SwissProt ID | Q15858 |
|---|
WB
Western blot analysis using NaV1.7 antibody against Mouse skin tissue(1) . 6% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:2000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.
ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.| Application Notes | WB:1:1000-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | PBS ONLY |
