WB
Western blot analysis using SMARCA1 antibody against K562(1),Huvec(2),C2C12(3),Hek293(4),Mcf-7(5),Jurkat(6)cell . 6% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:2000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.| Product Name | Anti-SMARCA1 antibody,pbs only |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| WB:1:1000-1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | SMARCA1 |
|---|---|
| Gene Synonyms | 5730494M04Rik hSNF2L ISWI NURF140 SNF2L SNF2L1 SNF2LB SNF2LT SWI SWI2 |
| Gene Full Name | SNF2 related chromatin remodeling ATPase 1 |
| Gene Summary | This gene encodes a member of the SWI/SNF family of proteins. The encoded protein is an ATPase which is expressed in diverse tissues and contributes to the chromatin remodeling complex that is involved in transcription. The protein may also play a role in DNA damage, growth inhibition and apoptosis of cancer cells. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013] |
| Alternative Names | ATP-dependent helicase SMARCA1 DKFZp686D1623 DNA-dependent ATPase SNF2L FLJ41547 global transcription activator homologous sequence global transcription activator SNF2L1 nucleosome-remodeling factor subunit SNF2L probable global transcription activator SNF2L1 RP3-353H6.2 SNF2-like 1 sucrose nonfermenting 2-like protein 1 SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily a member 1 SWI/SNF-related matrix-associated actin-dependent regulator of chromatin A1 truncated isoform of SNF2L |
| Molecular Weight(MW) | 123kDa(observed：130kDa) |
| Cellular Localization | Nucleus |
| SwissProt ID | P28370 |
|---|
WB
Western blot analysis using SMARCA1 antibody against K562(1),Huvec(2),C2C12(3),Hek293(4),Mcf-7(5),Jurkat(6)cell . 6% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:2000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.
ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.| Application Notes | WB:1:1000-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | PBS ONLY |
