WB
Western blot analysis using CD64 antibody against Cos-7(1) cell. 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:15000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.| Product Name | Anti-CD64 antibody,pbs only |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| WB:1:1000-1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Antigen peptide affinity chromatography |
| Gene Symbol | FCGR1A |
|---|---|
| Gene Synonyms | CD64 CD64A FCG1 FcgammaRI FCGR1 Fcgr1b FCRI IGFR1 IGGHAFC |
| Gene Full Name | Fc gamma receptor Ia |
| Gene Summary | This gene encodes a protein that plays an important role in the immune response. This protein is a high-affinity Fc-gamma receptor. The gene is one of three related gene family members located on chromosome 1. [provided by RefSeq, Jul 2008] |
| Alternative Names | CD64 antigen Fc fragment of IgG high affinity Ib receptor Fc fragment of IgG receptor Ia Fc fragment of IgG high affinity Ia receptor (CD64) Fc fragment of IgG high affinity Ia receptor for (CD64) Fc fragment of IgG high affinity Ib receptor for (CD64) Fc gamma receptor Ia Fc receptor IgG high affinity I Fc-gamma receptor 1 Fc-gamma receptor I A1 Fc-gamma RI fc-gamma RIA FcgammaRI fcgammaRIa FcgRI fcRI FLJ18345 high affinity IgG Fc receptor subunit beta high affinity IgG receptor high-affinity immunoglobulin gamma Fc receptor I IgG Fc receptor I IgG high affinity Fc receptor RP11-196G18.2 |
| Molecular Weight(MW) | 43kDa(observed：37kDa) |
| Cellular Localization | Cell membrane |
| SwissProt ID | P12314 |
|---|
WB
Western blot analysis using CD64 antibody against Cos-7(1) cell. 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:15000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.
ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.| Application Notes | WB:1:1000-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | PBS ONLY |
