WB
Western blot analysis using CACNA1B antibody against C2C12(1),Huvec(2),Mcf-7(3),Raji(4),A431(5)cell. 6% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:15000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.| Product Name | Anti-CACNA1B antibody,pbs only |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| WB:1:1000-1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | CACNA1B |
|---|---|
| Gene Synonyms | alpha(1B) BIII CACNL1A5 CACNN Cav2.2 Cchn1a DYT23 NEDNEH |
| Gene Full Name | calcium voltage-gated channel subunit alpha1 B |
| Gene Summary | The protein encoded by this gene is the pore-forming subunit of an N-type voltage-dependent calcium channel, which controls neurotransmitter release from neurons. The encoded protein forms a complex with alpha-2, beta, and delta subunits to form the high-voltage activated channel. This channel is sensitive to omega-conotoxin-GVIA and omega-agatoxin-IIIA but insensitive to dihydropyridines. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011] |
| Alternative Names | alpha1B-a brain calcium channel III CACHA1B calcium channel alpha12.2 subunit calcium channel L type alpha-1 polypeptide calcium channel voltage-dependent L type alpha 1B subunit calcium channel voltage-dependent N type alpha 1B subunit calcium channel voltage-dependent N type alpha 1B subunit BIII Cav2.2 voltage-gated Ca2+ channel ion channel CACNA1B Cav2 2 voltage gated N-type calcium channel Ca(v)2.2 voltage-dependent N-type calcium channel subunit alpha-1B-like voltage-gated calcium channel alpha subunit Cav2.2 voltage-gated calcium channel subunit alpha Cav2.2 |
| Molecular Weight(MW) | 262kDa |
| Cellular Localization | Membrane |
| SwissProt ID | Q00975 |
|---|
WB
Western blot analysis using CACNA1B antibody against C2C12(1),Huvec(2),Mcf-7(3),Raji(4),A431(5)cell. 6% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:15000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.
ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.| Application Notes | WB:1:1000-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | PBS ONLY |
