WB
Western blot analysis using Alpha-synuclein antibody against Mouse lung tissue(1),Rat kidney tissue(2),Rat spleen tissue(3),Rat testes tissue(4) . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Mouse IgG (H&L) - HRP（OM644366）, at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60SELISA
Coat the plate with immunizing antigen.Blue line： Antibody; Orange line: Isotype control antibody| Product Name | Anti-Alpha-synuclein antibody,pbs only |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| WB:1:1000-1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Sncaip |
|---|---|
| Gene Synonyms | SYPH1 2810407O15Rik 4933427B05Rik Sph1 SYPH1 |
| Gene Full Name | synuclein, alpha interacting protein (synphilin) |
| Gene Summary | Predicted to enable identical protein binding activity and ubiquitin protein ligase binding activity. Predicted to be involved in dopamine metabolic process; regulation of inclusion body assembly; and regulation of neurotransmitter secretion. Predicted to be located in cytoplasmic ribonucleoprotein granule and nucleoplasm. Is expressed in several structures, including alimentary system; branchial arch; cerebral cortex; genitourinary system; and ureter. Orthologous to human SNCAIP (synuclein alpha interacting protein). [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | alpha-synuclein-interacting protein MGC39814 Synphilin 1 |
| Molecular Weight(MW) | 15kDa（observed：18kDa) |
| Cellular Localization | Cytoplasm;Membrane;Nucleus;Synapse;Secreted;Cell projection, axon |
| SwissProt ID | P37840 |
|---|
WB
Western blot analysis using Alpha-synuclein antibody against Mouse lung tissue(1),Rat kidney tissue(2),Rat spleen tissue(3),Rat testes tissue(4) . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Mouse IgG (H&L) - HRP（OM644366）, at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S
ELISA
Coat the plate with immunizing antigen.Blue line： Antibody; Orange line: Isotype control antibody| Application Notes | WB:1:1000-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | PBS ONLY |
