Application
Immunohistochemical staining of human skeletal muscle using Caspase-7 antibody at 2 μg/mL.Application
Western blot analysis of Caspase-7 in human skeletal muscle cell lysate with Caspase-7 antibody at (A) 0.5, (B) 1, and (C) 2 μg/mL.| Product Name | Caspase-7 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Caspase-7 Antibody: MCH3, CMH-1, LICE2, CASP-7, ICE-LAP3, MCH3, Caspase-7, Apoptotic protease Mch-3 |
| Product description | Caspase-7 Antibody: Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens. The apoptotic subfamily can be further divided into initiator caspases, which are activated in response to death signals, and executioner caspases, which are activated by the initiator caspases and are responsible for cleavage of cellular substrates that ultimately lead to cell death. Caspase-7 is an executioner caspase that was identified based on its homology with caspases 1 and 3, as well as the C. elegans cell death protein CED-3. Alternative splicing of Caspase-7 mRNA results in the production of 3 distinct isoforms. Caspase-7 activity can be directly inhibited by XIAP expression.1) Martinon F and Tschopp J. Inflammatory caspases: linking an intracellular innate immune system to autoinflammatory diseases. Cell 2004; 117:561-74. |
| Immunogen | Caspase-7 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human Caspase-7. The immunogen is located within the last 50 amino acids of Caspase-7. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| Casp-7 antibody can be used for the detection of Caspase-7 by Western blot at 0.5 to 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CASP7 |
|---|---|
| Alternative Names | Caspase-7 Antibody: MCH3 CMH-1 LICE2 CASP-7 ICE-LAP3 MCH3 Caspase-7 Apoptotic protease Mch-3 |
| Tissue Specificity | Depending on cell lines or tissues used, other cleavage products may be observed. |
Application
Immunohistochemical staining of human skeletal muscle using Caspase-7 antibody at 2 μg/mL.
Application
Western blot analysis of Caspase-7 in human skeletal muscle cell lysate with Caspase-7 antibody at (A) 0.5, (B) 1, and (C) 2 μg/mL.| Positive Control | 1) Cat. No. 1375 - Human Skeletal Muscle Tissue Lysate |
|---|---|
| Application Notes | Casp-7 antibody can be used for the detection of Caspase-7 by Western blot at 0.5 to 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Caspase-7 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Caspase-7 Antibody is supplied in PBS containing 0.02% sodium azide. |
Data sheet for OM273517